FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317753
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT10640.3348512838588463No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCACG10610.33390715429909396No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC10510.330760055766586No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9730.30621268721302397No Hit
CCCTTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT9240.290791904403735No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8620.2712798935021857No Hit
GTGCTGGTGTGTGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAG8510.2678180851164269No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8180.25743265995915066No Hit
CATTTAAACCCCACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8170.2571179501058999No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATGTGTTCAGG7900.2486207840681284No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7570.23823535891085212No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6840.21526153962354408No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6750.21242915094428694No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG6610.2080232129987758No Hit
ATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTGTGTACAATATGTT6420.20204372578701066No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6210.19543481886874398No Hit
ATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAG6050.19039946121673124No Hit
GTATACAAGTGAGTCATTGACAAAACCTGCAGTGTGTGTGAACCCACTGG5690.17906990649970259No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT5610.17655222767369624No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAG5530.17403454884768987No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5490.17277570943468668No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5420.1705727404619311No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5260.1655373828099184No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5100.16050202515790565No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT5070.15955789559815328No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTGGGGTTTAAATGTATA4970.15641079706564534No Hit
GAGTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4870.15326369853313737No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4850.15263427882663577No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4770.15011660000062943No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC4710.14822834088112463No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTGTGTACAAT4630.1457106620551183No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4510.14193414381610875No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4510.14193414381610875No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4500.14161943396285792No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4480.14099001425635635No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4450.14004588469660398No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4380.1378429157238484No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4380.1378429157238484No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4310.13563994675109284No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4310.13563994675109284No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4290.13501052704459124No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4150.1306045890990801No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4110.12934574968607693No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4030.12682807086007056No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTC3990.1255692314470674No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC3910.12305155262106102No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTC3850.12116329350155625No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3840.12084858364830545No Hit
GGGTAACCACATTGTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAAT3820.12021916394180385No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG3820.12021916394180385No Hit
GTGCTGGTGGCTGCCACGCCTGGTCATCCTAGCCCACAGGCCCATGTAGT3790.11927503438205146No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3790.11927503438205146No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG3770.11864561467554988No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3740.11770148511579749No Hit
GGAACATATTGTACACAAAATCAAGCAATGTTTTCGGAAACTTCCTATAA3700.11644264570279432No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA3690.1161279358495435No Hit
GACGCAGAAGGGGACGGTAGAGTCCCAAACGGCCCCGAGACGGGTCGTCC3590.11298083731703557No Hit
TTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCG3550.1117219979040324No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3550.1117219979040324No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG3540.11140728805078158No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG3520.11077786834428No Hit
GCATTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATTTGGCCAC3490.10983373878452761No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTGTGT3460.10888960922477522No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3450.10857489937152442No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA3400.10700135010527044No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT3390.10668664025201965No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT3380.10637193039876885No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCAAGCGCTGATGGCC3380.10637193039876885No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3370.10605722054551805No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT3310.1041689614260133No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3250.10228070230650851No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC3240.10196599245325773No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG3190.10039244318700373No Hit
ATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAG3180.10007773333375294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)