FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467507
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT20460.43764050591755843No Hit
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATATGTTCAGG19730.42202576645911183No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG18370.39293529294748525No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13630.29154643673784564No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11800.25240263782146577No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG10990.23507669403880585No Hit
GTGTAAAGGGAGCGGCAAAACCTAAAAGACCCACAATTCTTTGACATACT10810.23122648430932585No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG10610.22694847349879252No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT10400.22245656214773252No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9570.2047028172840193No Hit
TCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATA9290.19871360214927264No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCACAT9200.19678849728453265No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT9080.19422169079821264No Hit
CATGTATACAAGCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8780.18780467458241265No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8730.18673517187977934No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8560.18309886269082604No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8390.1794625535018727No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC8040.1719760345834394No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT7820.16727022269185274No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7800.1668424216107994No Hit
GTGTAGCAGCTGAGGAATACAGATGGATAAGTTACGTTTTCTTCCCTCAA7740.16555901836763942No Hit
AGTCTGTACAACATCTTGAATCCCTTTATACCGCTATTACCAATTTTCTT7720.16513121728658609No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT7570.1619227091786861No Hit
GAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATATGTTCA7310.15636129512499278No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7300.1561473945844661No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7110.15208328431445947No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA6780.14502456647707948No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA6700.14331336215286616No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC6680.14288556107181283No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6570.1405326551260195No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6530.13967705296391283No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6500.13903535134233283No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA6500.13903535134233283No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA6440.13775194809917285No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA6440.13775194809917285No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC6310.1349712410723262No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGC6200.13261833512653284No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTATAA6110.1306932302617929No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGTACCTGTC6100.13047932972126622No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG5930.1268430205323129No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC5600.11978430269493291No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT5520.11807309837071958No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5440.11636189404650625No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCACATATTCATTTGAAAG4980.10652246918227962No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTTGGA4890.10459736431753963No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4880.10438346377701296No Hit
GTGCTGGTGGGAGTGAGAGGCCACTTAAGAGCAGCCACCAAATGGGAGAG4850.10374176215543296No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4770.10203055783121963No Hit
ACCTAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT4740.10138885620963964No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAG4680.10010545296647966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)