FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525361
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13830.26324755739386824No Hit
CATCTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9880.1880611617535371No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9630.1833025291180731No Hit
ATATAAGACTGCACCCCTATGACCATCTGATCTTCAACAAAGCTGACAAA8860.16864594060084398No Hit
ACTAACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8560.1629355814382872No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8150.15513142391612625No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGT7710.1467562304777096No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAAAGTCCAA7500.14275897906391985No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7240.13781000112303732No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTTGAAGTAATGATTAACTACT7180.13666792929052596No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6800.12943480768462068No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC5630.10716440695064917No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5540.10545129920188213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)