FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525361
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13770.2621054855613569No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT8820.16788455937916974No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8110.15437004269445198No Hit
CATCTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7410.1410458713151528No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6440.12258237668955252No Hit
ACTAACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6300.11991754241369268No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6300.11991754241369268No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTTGAAGTAATGATTAACTACT6260.11915616119201845No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAAAGTCCAA6250.11896581588659988No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGT5770.10982924122650901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)