FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513879
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10850.2111391981380831No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10140.19732271604794124No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC9600.18681440572586153No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9550.18584141402937268No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC9350.18194944724341724No Hit
CATGTATACAAGCTAAACAGGCTTTTACCTTCTCGCCAACTTACAAGTCC8820.1716357352606353No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8730.16988435020695533No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8560.1665761784388932No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT8140.15840304818838677No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8050.15665166313470683No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7720.15022991793788032No Hit
CATCTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7570.14731094284841373No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT7360.14322437772316052No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7130.13874861591931176No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7010.1364134358477385No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC6890.1340782557761652No Hit
GTAAACACACAGTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACATTGC6740.13115928068669863No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6690.13018628899020976No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA6590.12824030559723204No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6570.1278511089186365No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGCTGACATACT6490.1262943222042543No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6370.12395914213268104No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6280.1222077570790011No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6250.12162396206110777No Hit
GAATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6230.12123476538251222No Hit
CCTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT6170.12006717534672559No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAGCGAATTGTGGGTCT6110.11889958531093896No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6060.1179265936144501No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6030.11734279859655677No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6010.11695360191796124No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5920.11520221686428128No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACCTT5890.11461842184638796No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5580.10858587332815701No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5520.10741828329237038No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5310.10333171816711716No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5210.10138573477413944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)