FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522217
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGAAAGTAATGATTAACTATATGTTCAGG16400.3140456936484258No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG14270.27325805172945344No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC13110.25104506364212575No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA13010.24913015087597684No Hit
GTGTAAAAGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT12700.24319392130091513No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA11670.22347031980958107No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10950.20968294789330874No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10850.2077680351271598No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10490.2008743491690236No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG10150.19436364576411722No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC10010.1916827678915087No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT9540.18268267789060869No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9100.17425706171955338No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTAT8960.17157618384694484No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT8550.1637250415057342No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8500.16276758512265974No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG8410.1610441636331257No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8300.15893775959036185No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8260.15817179448390228No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC8040.1539589863983746No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC7960.15242705618545546No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA7300.13978863192887248No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7260.1390226668224129No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7010.13423538490704057No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC6970.133469419800581No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6320.1210224868206129No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6310.12083099554399801No Hit
AGTCTGTACAACATCTTGAATCCCTTTATACCGCTATTACCAATTTTCTT6210.11891608277784906No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC6060.11604371362862564No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC6010.11508625724555117No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6000.11489476596893627No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5990.11470327469232139No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5880.11259687064955756No Hit
CATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGAGCGGCA5850.11202239681971289No Hit
ATTGATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT5650.108192571287415No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5350.10244783298896819No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5320.10187335915912352No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGAAAGTAATGATTAACTATA5300.10149037660589372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)