FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265667
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14650.5514422190185457No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT10310.38807981420349535No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT9740.3666243831563574No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6900.25972363899167No Hit
TCCACAGAGTGTGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG6860.2582179947076603No Hit
ATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6720.25294823971362645No Hit
GTGTGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG6710.25257182864262406No Hit
GGATATTATAAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG6430.24203231865455627No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA5920.22283535403343283No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT5830.21944765439441105No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5460.2055204447673215No Hit
ATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC5450.20514403369631906No Hit
ATTAGTAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5380.20250915619930213No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGTTT5380.20250915619930213No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT5320.2002506897732876No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5310.1998742787022852No Hit
AAGGTGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA5250.19761581227627067No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT5180.1949809347792537No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT5070.1908404129982271No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTACTAAA5050.19008759085622226No Hit
GGATATCATAAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG4980.18745271335920533No Hit
ATTTATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC4880.1836886026491811No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC4770.1795480808681545No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4700.17691320337113756No Hit
TATCATAAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAA4640.174654736945123No Hit
ATTATAAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA4580.1723962705191085No Hit
GTGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT4450.16750292659607705No Hit
GTAGTAAGCTATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATT4220.15884547196302137No Hit
GTACAGGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA4180.1573398276790117No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG4160.15658700553700686No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC3990.1501880173299657No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTACTAAA3950.14868237304595602No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA3850.14491826233593183No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT3800.1430362069809197No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG3710.13964850734189796No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA3660.13776645198688584No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3590.1351315744898689No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA3510.13212028592184952No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3370.12685053092781565No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT3370.12685053092781565No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG3360.12647411985681323No Hit
GAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAACTGTAA3310.12459206450180112No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3280.12346283128879386No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3240.1219571870047842No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG3240.1219571870047842No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC3190.12007513164977207No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA3170.11932230950776725No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC3120.11744025415275515No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGT3110.11706384308175272No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3110.11706384308175272No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA3050.1148053766557382No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC3040.11442896558473577No Hit
GTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGGGGCTACT3010.11329973237172851No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC2990.11254691022972367No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA2960.11141767701671641No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC2920.10991203273270675No Hit
TTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC2910.10953562166170432No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC2900.1091592105907019No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA2880.10840638844869706No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT2860.1076535663066922No Hit
TACCTCGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2710.1020074002416559No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC2680.10087816702864866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)