FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265667
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14640.5510658079475433No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT9750.3670007942273598No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT8380.31543247750002823No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT7260.27327443754775715No Hit
GGATATTATAAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG7190.2706395600507402No Hit
ATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6590.24805489579059498No Hit
TCCACAGAGTGTGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG6070.22848152009846914No Hit
GTGTGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG6000.2258466426014522No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT5950.22396458724644008No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA5750.21643636582639167No Hit
ATTAGTAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5500.20702608905133119No Hit
GGATATCATAAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG5330.20062710084429006No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT5280.19874504548927793No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGTTT5270.19836863441827549No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5160.19422811263724887No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5090.19159323514023197No Hit
AAGGTGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA5030.18933476871421742No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4940.18594706907519565No Hit
ATTTATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC4940.18594706907519565No Hit
GTGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT4920.1851942469331908No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4900.18444142479118597No Hit
ATTATAAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA4870.1833121915781787No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT4830.181806547294169No Hit
TATCATAAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAA4810.18105372515216417No Hit
GTACAGGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA4780.1799244919391569No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC4710.17728961444213998No Hit
ATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC4520.17013780409309398No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTACTAAA4370.16449163802805766No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG4080.1535757169689875No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC3960.14905878411695844No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT3820.14378902912292457No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTACTAAA3780.14228338483891487No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA3760.14153056269691006No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG3730.14040132948390277No Hit
GTAGTAAGCTATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATT3700.1392720962708955No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG3660.13776645198688584No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3520.13249669699285196No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3510.13212028592184952No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3410.12835617521182532No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT3380.12722694199881807No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGT3360.12647411985681323No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA3320.12496847557280355No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3250.1223335980757866No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA3210.12082795379177692No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3210.12082795379177692No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA3110.11706384308175272No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA3110.11706384308175272No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA3100.1166874320107503No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC3090.11631102093974788No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC3070.11555819879774305No Hit
GTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGGGGCTACT3060.11518178772674062No Hit
TGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT2910.10953562166170432No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG2910.10953562166170432No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC2880.10840638844869706No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG2850.1072771552356898No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC2840.10690074416468737No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT2830.10652433309368495No Hit
GTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATAC2810.10577151095168011No Hit
GAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAACTGTAA2810.10577151095168011No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC2780.10464227773867285No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG2770.10426586666767042No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA2720.10238381131265832No Hit
TTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC2700.1016309891706535No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT2700.1016309891706535No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2700.1016309891706535No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC2660.1001253448866438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)