FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624689
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT26440.42325060950328885No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA17020.27245557389356945No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT16390.26237055558846084No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA15030.2405997224218771No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA14190.22715303134839895No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT13890.2223506416792996No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG13720.21962928753347669No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12850.20570235749308857No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12810.20506203887054197No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12810.20506203887054197No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12740.2039414812810855No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12670.20282092369162896No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA12390.1983386933338029No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA12350.19769837471125631No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC12190.19513710022107No Hit
GGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA12080.1933762240090669No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTATGTGCCAAGTG11900.19049479020760732No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11890.19033471055197065No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT11460.18345128535959493No Hit
ATGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA11270.18040977190249868No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT10320.16520220461701743No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT10130.16216069115992118No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10020.16039981494791808No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT9770.15639782355700196No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA9580.15335631009990572No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT9430.15095511526535604No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8650.13846890212569773No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT8610.13782858350315116No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8550.13686810556933132No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT8340.13350643280096178No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT8110.12982460072131893No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8020.12838388382058913No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7930.12694316691985932No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7800.12486213139658293No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7800.12486213139658293No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC7720.12358149415148978No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7710.12342141449585313No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA7610.12182061793948669No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCATCCCAACAGTCCCCCATG7580.12134037897257674No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7430.11893918413802707No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7410.1186190248267538No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7330.11733838758166064No Hit
GCACCGGACCATGCAAAACCTGCACAGCTCCTGCTCAAGGAACCTCTATG7280.11653798930347742No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG7240.11589767068093083No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7200.11525735205838426No Hit
GGGTAAAGGTTTAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7180.11493719274711096No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG7090.11349647584638116No Hit
CCTTCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6980.11173559963437807No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6940.11109528101183148No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6890.11029488273364826No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6890.11029488273364826No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6860.10981464376673834No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6760.10821384721037189No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6680.10693320996527872No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG6580.10533241340891228No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCTCCAAACTCTTCAAG6460.10341145754127254No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6410.10261105926308932No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6370.10197074064054275No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG6310.10101026270672286No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAGGCTTTTACTTT6290.10069010339544958No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6270.1003699440841763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)