FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497970
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26390.5299516035102516No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG20260.40685181838263346No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT18680.3751229993774725No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18270.36688957166094344No Hit
TTATTAATAGATGTCAACAATACGTGGGCCCTCTTACAATTAATGAAAAA17960.3606642970460068No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17570.35283249994979615No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG14700.2951985059340924No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA14160.2843544791854931No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT14040.28194469546358214No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT12220.24539630901459927No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11860.2381669578488664No Hit
TTATTGATAGATGTCAACAATACGTGGGCCCTCTTACAATTAATGAAAAA10930.21949113400405645No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTTTGACATACT10570.2122617828383236No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT10340.20764303070466092No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10320.20724140008434241No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10040.2016185713998835No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9970.2002128642287688No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9850.19780308050685785No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9820.1972006345763801No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT9710.19499166616462837No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9140.18354519348555134No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA9140.18354519348555134No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA8850.1777215494909332No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8680.174307689218226No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8590.1725003514267928No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8210.1648693696407414No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8070.16205795529851194No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8050.16165632467819346No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7930.1592465409562825No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATG7660.15382452758198284No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATT7610.1528204510311866No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7590.15241882041086813No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7560.15181637448039037No Hit
GAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7410.1488041448280017No Hit
CGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCT7370.1480008835873647No Hit
GTCTATTTACAGGCAATTTGCGAAAACATTGCTTGAGTTTTAGTACAATA7370.1480008835873647No Hit
TATTAATAGATGTCAACAATACGTGGGCCCTCTTACAATTAATGAAAAAA7310.14679599172640923No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA7270.14599273048577224No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7260.145791915175613No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC7230.14518946924513526No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7180.14418539269433903No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7100.14257887021306503No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7030.14117316304195032No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6980.1401690864911541No Hit
GGTCTATTTACAGGCAATTTGCGAAAACATTGCTTGAGTTTTAGTACAAT6970.13996827118099484No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTGCGAAAAC6870.13796011807940237No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC6690.13434544249653593No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6690.13434544249653593No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC6600.13253810470510272No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATTTACAT6590.13233728939494346No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC6510.1307307669136695No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCGGC6470.12992750567303252No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6450.129525875052714No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6420.12892342912223628No Hit
ATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT6370.12791935257144005No Hit
ACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGC6310.1267144607104846No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6280.12611201478000683No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6280.12611201478000683No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6230.1251079382292106No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6180.12410386167841436No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAT6000.12048918609554793No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5960.11968592485491095No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5950.1194851095447517No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5890.11828021768379622No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCA5830.11707532582284072No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5800.116472879892363No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5760.115669618651726No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5670.11386228086029278No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5660.11366146555013355No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5590.11225575837901883No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5560.11165331244854107No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5510.11064923589774484No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5430.10904271341647089No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5410.1086410827961524No Hit
CAATAGGTCTATTTACAGGCAATTTGCGAAAACATTGCTTGAGTTTTAGT5400.10844026748599313No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5390.10823945217583388No Hit
ATTTACAGGCAATTTGCGAAAACATTGCTTGAGTTTTAGTACAATATGTT5380.10803863686567464No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5380.10803863686567464No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATGC5320.10683374500471915No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATTAGA5300.10643211438440066No Hit
ACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTT5290.10623129907424142No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5220.1048255919031267No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5210.10462477659296744No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5210.10462477659296744No Hit
GATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGC5190.10422314597264895No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5090.10221499287105648No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5080.10201417756089724No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5050.1014117316304195No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5010.10060847038978252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)