FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366241
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG22620.6176260986618101No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT18650.5092275305058691No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17310.4726396007000855No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG14470.3950950330520067No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG14100.38499239571757393No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG13310.36342189978729855No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT13110.3579610147416592No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT12730.34758533315494444No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC12460.3402131383433313No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12280.3352983418022559No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11060.30198694302385587No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG9220.25174680060397386No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAAAGGACAACAGAGT8680.23700241098074765No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8530.23290674719651816No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA8220.22444237537577716No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8210.22416933112349519No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC8080.22061975584382962No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7780.2124284282753706No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT7750.2116092955185247No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT7720.21079016276167878No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG7640.20860580874342305No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7570.20669449897744926No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7350.200687525427246No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA7260.19823012715670826No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA7080.19331533061563289No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT7040.19222315360650502No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6940.18949271108368534No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6870.18758140131771156No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC6800.18567009155173778No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6750.18430487029032797No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT6650.1815744277675083No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6630.18102833926294434No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6500.17747876398327878No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6400.1747483214604591No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6310.1722909231899214No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG6230.17010656917166564No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC6150.1679222151534099No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6120.16710308239656402No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6060.1654648168828722No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTATAA5870.16027697608951483No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5830.15918479908038696No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC5810.15863871057582302No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5780.15781957781897712No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5720.15618131230528534No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5690.15536217954843942No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAACCTCTATG5660.1545430467915935No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5640.15399695828702958No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5640.15399695828702958No Hit
GGTCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT5600.1529047812779017No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5590.15263173702561975No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5320.14525954221400664No Hit
CACTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5260.14362127670031483No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5160.14089083417749515No Hit
GGGTATCCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAAT5100.13925256866380334No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC5060.13816039165467547No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5030.13734125889782955No Hit
CTTGTATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4940.13488386062729185No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT4890.13351863936588204No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4850.13242646235675418No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC4790.13078819684306237No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA4760.12996906408621645No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG4710.1286038428248066No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4630.12641948880655088No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT4470.1220507807700394No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4450.12150469226547547No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4380.11959338249950169No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC4380.11959338249950169No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4370.11932033824721973No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4350.1187742497426558No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4350.1187742497426558No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGT4340.11850120549037382No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4340.11850120549037382No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA4330.11822816123809186No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4310.11768207273352793No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4270.11658989572440005No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4260.11631685147211808No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4260.11631685147211808No Hit
GTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4210.11495163021070826No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCTAAAAC4200.1146785859584263No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4190.11440554170614431No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4100.11194814343560662No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTAAAAG4070.1111290106787607No Hit
GTGGATACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG4070.1111290106787607No Hit
GGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAACCTCTATGTTTCC4040.11030987792191481No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4020.10976378941735088No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC4000.10921770091278694No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC3990.10894465666050497No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA3970.10839856815594102No Hit
CAATAGGTCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGT3950.10785247965137709No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG3920.1070333468945312No Hit
TGCAAGACCTGCACGAGTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3910.10676030264224923No Hit
GTATCCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTC3890.1062142141376853No Hit
CCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGG3880.10594116988540334No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT3880.10594116988540334No Hit
GGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTTGCGAAAGCCCAAG3860.10539508138083939No Hit
CCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3790.10348377161486563No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA3750.10239159460573774No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3750.10239159460573774No Hit
CTACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA3700.10102637334432792No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC3680.10048028483976398No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT3670.10020724058748202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)