FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628891
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN103131.6398708202216283No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC23910.3801930700232632No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT21150.33630629155131814No Hit
ATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGAT19980.31770211372081963No Hit
CCCTTAACTTCATGGGATATATAATTGGAAGTTGGGGTACTTTACCGCAA18200.28939832180775366No Hit
ATATAATTGGAAGTTGGGGTACTTTACCGCAAGATCATATTGTACTAAAA17750.2822428687960235No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17450.27747256678820337No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAA16600.2639567110993797No Hit
GTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA16390.26061749969390563No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16260.25855036882385024No Hit
CTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGTTT16250.25839135875692293No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT14880.2366069795878777No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA14790.23517588898553168No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13880.220705972895144No Hit
ATATGATCTTGCGGTAAAGTACCCCAACTTCCAATTATATATCCCATGAA13370.2125964594818498No Hit
ATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTACCTGTAAAT12850.20432793600162827No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGTAGTTTTCGAAAAC12600.20035268432844483No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA12440.19780852325760745No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT12340.19621842258833408No Hit
CTCCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGA12280.19526436218677004No Hit
CAATATGATCTTGCGGTAAAGTACCCCAACTTCCAATTATATATCCCATG12260.19494634205291536No Hit
GGATATATAATTGGAAGTTGGGGTACTTTACCGCAAGATCATATTGTACT11960.19017604004509525No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11850.18842692930889454No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT11710.1862007883719118No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11610.18461068770263847No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA11510.18302058703336507No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11410.1814304863640917No Hit
TATATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG11210.17825028502554496No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA11200.17809127495861762No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC11140.1771372145570536No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11080.1761831541554896No Hit
ATACTATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11080.1761831541554896No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA10890.17316196288387017No Hit
ATGTGGTATTGGGGGCTAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10500.16696057027370403No Hit
CAATTATATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA10160.16155422799817457No Hit
GGTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT10090.16044115752968321No Hit
CAATAGGTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTAGT10020.15932808706119184No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9990.15885105686040982No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9800.15582986558879042No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGT9640.15328570451795304No Hit
CATATAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAAGGGGCAGCA9620.15296768438409836No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA9340.14851540251013293No Hit
AGATCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTACCTGTAA9330.1483563924432056No Hit
CTTCAAAGACTGTGTGTTTAAGGACTGGGAGGAGTTAGGGGAGGAGATTA9000.14310906023460346No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGGAGGGAGTTTG8760.13929281862834736No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG8660.137702717959074No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC8470.1346815266874546No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8310.13213736561661718No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8150.1295932045457798No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATATCCACAT8100.12879815421114313No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT8010.12736706360879707No Hit
CTTTTACACAATGTGGATATCCTGCCTTGATGCCTTTATATGCATGTATA7950.12641300320723306No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT7930.12609498307337838No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATATCCACATTGTGT7910.1257769629395237No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGC7820.12434587233717766No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7730.12291478173483163No Hit
ATATAGTATTGGGGGCTAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7560.12021161059706689No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7540.11989359046321221No Hit
ATTATATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATT7530.11973458039628489No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT7290.11591833879002879No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7220.11480526832153744No Hit
GTACAATATGATCTTGCGGTAAAGTACCCCAACTTCCAATTATATATCCC7210.1146462582546101No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC7200.11448724818768276No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCT7120.11321516765226407No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6920.11003496631371733No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6900.10971694617986265No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC6750.10733179517595258No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCTTTT6640.10558268443975188No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACTGCACTCAGGCA6640.10558268443975188No Hit
GCACGACTACTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6510.1035155535696965No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6500.10335654350276915No Hit
GCCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTAT6480.10303852336891449No Hit
TTTTAGTACAATATGATCTTGCGGTAAAGTACCCCAACTTCCAATTATAT6460.10272050323505981No Hit
ATGTGGATATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGC6420.10208446296735046No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6350.10097139249885911No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA6290.10001733209729508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)