FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307364
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT13710.4460509363490845No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11930.3881391444671464No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9360.30452492809828086No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8230.2677607006676124No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8210.26711000637680404No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7910.25734959201467966No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7900.2570242448692755No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7010.2280683489283065No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTT6940.22579091891047745No Hit
TGATTAACTACATGCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG6580.21407842167592822No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6250.2033419658775914No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6220.20236592444137896No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTTTTGTC5770.1877253028981924No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5740.18674926146197993No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5560.1808930128447053No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5530.17991697140849286No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5440.17698884709985555No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5380.17503676422743067No Hit
CCTTTATGCCGCTGTTACCCATTTTCTTTTGTCTTTGGGTATACATTTAA5200.16918051561015604No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5190.1688551684647519No Hit
GTTCCGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA5110.16625239130151873No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5040.16397496128368969No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4940.16072148982964823No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4870.1584440598118192No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4670.1519371169037363No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA4610.14998503403131141No Hit
CTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTG4590.1493343397405031No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4550.14803295115888654No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4270.13892323108757043No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT4260.1385978839421663No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4230.13762184250595386No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4230.13762184250595386No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG4220.1372964953605497No Hit
ATATTAACGTTGACATAACTGACTACTAATTCCCTGGATGCTGGATCTTC4090.1330669824702958No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4080.13274163532489167No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4080.13274163532489167No Hit
ACTACATGCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA4030.13111489959787093No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4000.1301388581616585No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA3970.12916281672544605No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3940.1281867752892336No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT3880.12623469241680874No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3840.12493330383519215No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG3820.12428260954438386No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3700.12037844379953411No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3680.11972774950872582No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA3680.11972774950872582No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG3670.11940240236332166No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG3650.11875170807251337No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA3620.11777566663630093No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3590.11679962520008849No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3560.11582358376387605No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3520.11452219518225948No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3520.11452219518225948No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT3510.11419684803685533No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG3450.11224476516443044No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT3440.11191941801902631No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAATCCCCCATG3370.10964198800119727No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC3360.10931664085579312No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA3340.10866594656498484No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG3290.10703921083796411No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT3280.10671386369255995No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT3270.10638851654715582No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3270.10638851654715582No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTTTTG3270.10638851654715582No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3240.10541247511094338No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT3240.10541247511094338No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC3180.1034603922385185No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3160.10280969794771021No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGG3160.10280969794771021No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3150.10248435080230606No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT3140.10215900365690192No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAA3130.10183365651149777No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3130.10183365651149777No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3130.10183365651149777No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG3130.10183365651149777No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3120.10150830936609362No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3100.10085761507528533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)