FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711707
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49110.6900311504593885No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG46890.658838538893112No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT33600.47210439127337517No Hit
CTCTTACAGTTAACGAAACAAGGAGATTAAAATTGATTATGCCTGCTAGG23920.3360933642636647No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAACAAGGAGATTAAAATTGA20800.29225509935970845No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA20700.2908500267666329No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT19400.2725840830566511No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTGTTTCG19260.2706169814263454No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG18840.2647156765354282No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC18530.2603599514968941No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT17820.25038393608605786No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT17160.24111045697175945No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT16530.23225849963538367No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16390.23029139800507795No Hit
ATGTAGGCCCTCTTACAGTTAACGAAACAAGGAGATTAAAATTGATTATG15190.21343052688817168No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14930.2097773381461753No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA14520.20401654051456566No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14220.19980132273533913No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC14130.19853675740157117No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAACAAGGA13180.1851885677673537No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13010.18279994435912533No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA12970.1822379153218951No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC12820.18013030643228184No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAACAA12780.1795682773950516No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC12740.1790062483578214No Hit
GATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGA12640.1776011757647459No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT12610.17717965398682323No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12510.17577458139374771No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTGTTTCGTTAAC12510.17577458139374771No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT11600.16298842079676046No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC10530.1479541440508524No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10400.14612754967985422No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC10140.14247436093785787No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC9900.1391021867144766No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAACAAGGAGATTAAAATTGATTA9770.13727559234347841No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9720.13657305604694067No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCTTCTTTC9700.13629204152832555No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9650.1355895052317878No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT9490.13334138908286697No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAC9430.13249834552702167No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT9260.13010972211879326No Hit
CAATTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT9150.1285641422664102No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8810.12378689544995342No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA8760.12308435915341566No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA8750.12294385189410811No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8590.12069573574518729No Hit
ACATATTGTTGACATCTATTGATAATATCTTCTTTCAAATGAATGTGAGG8570.12041472122657218No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8450.11872863411488155No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT8390.11788559055903623No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCTTCTTTCAAATGAATG8370.11760457604042113No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8260.11605899618803805No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG8210.1153564598915003No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA8170.11479443085427009No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACC8100.11381088003911723No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA8100.11381088003911723No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA8050.11310834374257946No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7980.1121247929274266No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT7940.11156276389019638No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7910.11114124211227373No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC7780.10931464774127556No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT7770.109174140481968No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7570.10636399529581696No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG7510.10552095173997164No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7490.10523993722135654No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC7460.10481841544343387No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7300.10257029929451306No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACC7210.10130573396074508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)