FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711707
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG57670.8103053644266531No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49020.6887665851256206No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT27620.388081050207459No Hit
CTCTTACAGTTAACGAAACAAGGAGATTAAAATTGATTATGCCTGCTAGG23790.3342667698926665No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA20150.28312212750471755No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT19910.2797499532813363No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT19290.2710385032042681No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAACAAGGAGATTAAAATTGA19020.2672448072029641No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTGTTTCG18230.2561447337176675No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG17420.24476364571375578No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT17320.2433585731206803No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC17310.24321806586137273No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17060.2397053843786839No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA16360.22986987622715527No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT15700.22059639711285683No Hit
ATGTAGGCCCTCTTACAGTTAACGAAACAAGGAGATTAAAATTGATTATG14580.204859584070411No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14450.20303298969941283No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC14160.1989582791794938No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13650.19179240895480865No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13520.18996581458381046No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA13300.18687465487904434No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC13260.18631262584181413No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAACAA12810.17998979917297428No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC12790.17970878465435916No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAACAAGGA12340.17338595798551934No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC12260.1722618999110589No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTGTTTCGTTAAC12120.17029479828075317No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12000.16860871116906254No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT11760.1652365369456813No Hit
GATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGA11700.16439349338983597No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11620.16326943531537558No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10970.15413646346038468No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10180.14303638997508805No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC10180.14303638997508805No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC10150.1426148681971654No Hit
CAATTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT10110.1420528391599352No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAC9910.13924269397378417No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA9780.13741609960278597No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT9640.13544899797248025No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9600.13488696893525004No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAACAAGGAGATTAAAATTGATTA9550.1341844326387123No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCTTCTTTC9230.1296882003408706No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT9080.12758059145125733No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA8660.12167928656034015No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT8550.12013370670795706No Hit
ACATATTGTTGACATCTATTGATAATATCTTCTTTCAAATGAATGTGAGG8500.11943117041141932No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT8480.1191501558928042No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT8450.11872863411488155No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG8390.11788559055903623No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8370.11760457604042113No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8370.11760457604042113No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA8170.11479443085427009No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8160.11465392359496253No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG8150.11451341633565498No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCTTCTTTCAAATGAATG8130.11423240181703986No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7950.11170327114950393No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACC7860.11043870581573596No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG7830.11001718403781333No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7820.10987667677850577No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7780.10931464774127556No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7740.10875261870404533No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7740.10875261870404533No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC7650.10748805337027736No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC7480.10509942996204899No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7390.10383486462828102No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7310.1027108065538206No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA7170.10074370492351487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)