FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412394
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA19500.47284878053511936No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT16840.4083473571390466No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG16480.399617841190706No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14350.3479682051630237No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG13210.3203247379932783No Hit
CATCTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT13070.31692992623559024No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG12640.30650300440840555No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC12440.30165327332599406No Hit
ATCCAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11550.280071970009263No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT11500.27885953723866014No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGACGTTCACGGTGGTCTCCATGC10310.2500036372983118No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9800.23763682303816255No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG9230.22381508945328982No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9060.21969281803324003No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8690.21072081553077882No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8660.2099933558684171No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8520.20659854411072906No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8510.20635605755660846No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG8400.20368870546128218No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT8170.19811151471650898No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8000.1939892432964592No Hit
CTTCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATT7940.19253432397173575No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC7680.18622967356460082No Hit
CATTTGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7680.18622967356460082No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7600.18428978113163624No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7560.18331983491515397No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT7540.1828348618069128No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7510.1821074021445511No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7250.17580275173741616No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7140.17313539964208985No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7120.17265042653384868No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6980.16925561477616066No Hit
GCACGGGACCATGCAAAACCTGCACGATTCCTGCTCAAGGAACCTCTATG6840.1658608030184726No Hit
GGGAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA6810.1651333433561109No Hit
ACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGC6730.1631934509231463No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6640.16101107193606115No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC6620.16052609882782No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCAAGAAGATTGACGATATGGGA6620.16052609882782No Hit
TCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6570.1593136660572171No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6550.15882869294897597No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG6460.15664631396189083No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6330.15349398875832337No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6310.1530090156500822No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6180.14985669044651473No Hit
TCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6150.14912923078415302No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6120.1484017711217913No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6090.14767431145942958No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6020.14597690558058554No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT6010.145734419026465No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA5990.14524944591822384No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5860.14209712071465638No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5840.14161214760641522No Hit
GGGATTTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA5830.14136966105229465No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5750.13942976861933007No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5660.1372473896322449No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5520.13385257787455687No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5500.1333676047663157No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5480.13288263165807454No Hit
GTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5400.13094273922510996No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5370.13021527956274825No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5330.12924533334626595No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGACGTTC5290.12827538712978365No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGTCCCACAATTCGTTGACATACT5230.12682046780506023No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5210.12633549469681907No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5180.12560803503445733No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5160.1251230619262162No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5150.12488057537209563No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5140.12463808881797506No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTTTTGCATGGTCCCGTG5120.12415311570973389No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5120.12415311570973389No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC5090.12342565604737217No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5090.12342565604737217No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAGATGTATA5040.12221322327676931No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5010.12148576361440758No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA4980.12075830395204587No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC4950.12003084428968414No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4940.11978835773556355No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4930.11954587118144298No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA4930.11954587118144298No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4920.1193033846273224No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4920.1193033846273224No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4880.11833343841084012No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4880.11833343841084012No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4840.11736349219435781No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4830.11712100564023725No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTAAGGCA4780.11590857286963438No Hit
CTACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA4770.1156660863155138No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT4770.1156660863155138No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4750.11518111320727266No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4730.11469614009903151No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4720.11445365354491094No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4710.11421116699079036No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG4690.1137261938825492No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4640.11251376111194634No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4610.11178630144958461No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4610.11178630144958461No Hit
CCCAAAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAAC4600.11154381489546406No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4600.11154381489546406No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4580.1110588417872229No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG4550.11033138212486117No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4550.11033138212486117No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA4440.10766403002953487No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4440.10766403002953487No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA4430.1074215434754143No Hit
CCTCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGAT4380.10620911070481141No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCAAGAAGATTGACGATATGG4380.10620911070481141No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4340.10523916448832912No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4330.10499667793420855No Hit
GCTGATACTGTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAG4300.10426921827184682No Hit
CTAAATCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCC4280.10378424516360568No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4260.10329927205536453No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4250.10305678550124396No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4230.10257181239300281No Hit
GTATGGTGAGGTGAGCAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG4200.10184435273064109No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4140.10038943340591763No Hit
GCCTCCCAGTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCC4140.10038943340591763No Hit
CTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG4130.10014694685179706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)