FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412394
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT16970.4114996823426141No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG16890.40955978990964953No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16870.40907481680140834No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13700.3322065791451864No Hit
CATCTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT12470.30238073298835577No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11640.28225434899634816No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11430.2771621313598161No Hit
ATCCAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11120.2696450481820783No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT10690.25921812635489366No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10440.2531559625018793No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9920.24054666168760944No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG9140.22163271046620464No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT8860.2148430869508286No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8510.20635605755660846No Hit
CTTCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATT8500.20611357100248792No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8450.20490113823188505No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGACGTTCACGGTGGTCTCCATGC8380.20320373235304104No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8290.20102135336595586No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8240.199808920595353No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG8040.1949591895129415No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7990.19374675674233863No Hit
CATTTGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7990.19374675674233863No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7710.18695713322696256No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7660.1857447004563597No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7510.1821074021445511No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT7300.17701518450801904No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT7240.1755602651832956No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC7160.173620372750331No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6940.16828566855967836No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6800.16489085680199034No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6760.16392091058550803No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6730.1631934509231463No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6680.16198101815254343No Hit
TCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6670.16173853159842286No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC6370.15446393497480565No Hit
GGGAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA6330.15349398875832337No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6170.1496142038923942No Hit
GCACGGGACCATGCAAAACCTGCACGATTCCTGCTCAAGGAACCTCTATG6150.14912923078415302No Hit
TCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6110.14815928456767072No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6110.14815928456767072No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5930.1437945265935004No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5910.14330955348525926No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCAAGAAGATTGACGATATGGGA5890.1428245803770181No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG5880.14258209382289752No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA5730.1389447955110889No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5720.13870230895696833No Hit
ACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGC5580.13530749719928029No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT5490.13312511821219514No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5410.13118522577923053No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5380.13045776611686882No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5360.12997279300862769No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5310.12876036023802478No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5280.12803290057566308No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5260.12754792746742194No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5200.1260930081426985No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5170.12536554848033676No Hit
GGGATTTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA5140.12463808881797506No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAGATGTATA5140.12463808881797506No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5130.12439560226385447No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5100.12366814260149274No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGTCCCACAATTCGTTGACATACT5090.12342565604737217No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5070.12294068293913103No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4980.12075830395204587No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4940.11978835773556355No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4910.11906089807320185No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4890.1185759249649607No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTAAGGCA4860.11784846530259896No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4830.11712100564023725No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4820.11687851908611668No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG4790.11615105942375495No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4770.1156660863155138No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4760.11542359976139321No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4740.11493862665315209No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4700.11396868043666979No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG4640.11251376111194634No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA4610.11178630144958461No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4580.1110588417872229No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4570.11081635523310232No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4550.11033138212486117No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTTTTGCATGGTCCCGTG4540.1100888955707406No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA4540.1100888955707406No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4530.10984640901662002No Hit
CTACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA4520.10960392246249946No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4520.10960392246249946No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4510.10936143590837889No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4490.10887646280013774No Hit
CTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG4440.10766403002953487No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4430.1074215434754143No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4420.10717905692129372No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4420.10717905692129372No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT4410.10693657036717315No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA4340.10523916448832912No Hit
CTAAATCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCC4330.10499667793420855No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4330.10499667793420855No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4330.10499667793420855No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC4290.10402673171772624No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4270.10354175860948511No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4270.10354175860948511No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCAAGAAGATTGACGATATGG4270.10354175860948511No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4250.10305678550124396No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4250.10305678550124396No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4230.10257181239300281No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG4230.10257181239300281No Hit
GTATGGTGAGGTGAGCAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG4200.10184435273064109No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4200.10184435273064109No Hit
CCTCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGAT4190.10160186617652052No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4180.10135937962239994No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4160.10087440651415878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)