FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432390
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG29650.6857235366220311No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG21700.5018617451837462No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT20130.4655519322833553No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT16910.3910821249335091No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16280.37651194523462617No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG15900.36772358287656975No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG15680.3626355836166424No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14260.32979486112074746No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT14020.32424431647355395No Hit
CCCTTTACACAATGTGGGTACCCAGCATTAATGCCTTTGTATGCATGTAT13770.3184624991327274No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA12990.3004232290293485No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12330.2851592312495663No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11820.27336432387428017No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC10780.24931196373644163No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA10240.23682323828025628No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10070.23289160248849416No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT9780.22618469437313535No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT9570.22132796780684103No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA9530.22040287703230882No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9390.21716505932144592No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC9250.21392724161058305No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT8980.20768287888249035No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA8930.20652651541432504No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGGACCTTGCCACAG8890.20560142463979278No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8580.19843197113716782No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTACCCACATTGTGTAAAG8530.19727560766900254No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8120.18779342723004694No Hit
ATATGTTCCTGTGGCAAGGTCCCCCAACTTCCAATTACATATCCCATGAA8010.18524942760008326No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC8000.1850181549064502No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA7790.18016142834015586No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7750.17923633756562363No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC7580.17530470177386157No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACCGGG7440.1720668840629987No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7320.16929161173940194No Hit
GGAACATATTGTACAAAAAATCAAGCAATGTTTTCGGAAACTTCCTATAA7160.16559124864127292No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT6800.15726543167048265No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6650.15379634126598674No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6530.15102106894238998No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6360.1470894331506279No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6360.1470894331506279No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC6320.14616434237609566No Hit
ATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATATGTT6320.14616434237609566No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA6310.1459330696824626No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6260.14477670621429728No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6150.1422327065843336No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6100.14107634311616826No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTTCTACTG6090.14084507042253522No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA6000.13876361617983765No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5940.13737598001803927No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5920.13691343463077313No Hit
CTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTTCTACTGTACCTGTC5870.13575707116260785No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC5860.13552579846897475No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAAAG5820.13460070769444252No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5800.1341381623071764No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5770.1334443442262772No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5760.13321307153264414No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5750.13298179883901107No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5670.13113161728994657No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5580.129050163047249No Hit
CCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG5580.129050163047249No Hit
GTACCCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTC5380.12442470917458776No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5370.1241934364809547No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5360.12396216378732162No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAG5320.12303707301278939No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT5300.12257452762552326No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5210.1204930733828257No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5190.12003052799555956No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA5130.11864289183376119No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTC5120.11841161914012814No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5060.11702398297832974No Hit
GATTAACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGC4980.11517380142926524No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCTGGGTACCCACATTGTGT4930.11401743796109994No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4870.11262980179930154No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATGA4850.11216725641203544No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4850.11216725641203544No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4840.11193598371840235No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4770.11031707486297093No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTAT4750.1098545294757048No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4740.10962325678207174No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4730.10939198408843867No Hit
ATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT4720.10916071139480561No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC4570.10569162099030968No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCT4480.1036101667476121No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4450.10291634866671291No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG4430.10245380327944681No Hit
ATCCCAGAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGA4390.10152871250491455No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGGAT4390.10152871250491455No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAAATCTCCTCCC4380.10129743981128149No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTTTGTACAAT4360.10083489442401536No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAA4350.1006036217303823No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4340.10037234903674923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)