FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605899
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58090.9587406481938409No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG19020.3139137050894621No Hit
GTATAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC15760.26010935816035347No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15710.25928413811542844No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCATAC15160.2502067176212537No Hit
ATATTATACTAAAGCTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA12540.2069651872671848No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11880.1960722826741751No Hit
GTATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGAT11250.18567451010812033No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10990.18138336587451043No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10190.16817984515571077No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9940.16405374493108588No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9430.15563650047285107No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9420.1554714564638661No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC9200.15184048826619617No Hit
GTTCAGGATAGTATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA8910.1470542120056313No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8820.14556881592476634No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC8710.1437533318259314No Hit
AAACCATACTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCA8310.13715157146653154No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT8190.1351710433587116No Hit
CTGTAAGAGGGCCCACATATTGTTCACATCTATTAATAATGTCCTCCTGC8080.13335555925987663No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACATCTATTACCAATTTTCTT8040.13269538322393667No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA7740.12774406295438678No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6920.11421045421761712No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTTCGCCCCAAAAGACCGCCGTG6790.11206488210081218No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGCTTTAGTATAATA6730.1110746180469022No Hit
GTACAACATCTTGAGTCCCTTTTTACATCTATTACCAATTTTCTTTTGTC6680.11024939800197724No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA6560.10826886989415728No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC6530.10777373786720228No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG6360.10496798971445737No Hit
CTACTGTACCTGTCTTTAATTCTGAGTGGCAAACTCCCTCCTTTCCTCAC6280.1036476376425774No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACA6090.10051180147186246No Hit
TTCTTCTACTGTACCTGTCTTTAATTCTGAGTGGCAAACTCCCTCCTTTC6070.10018171345389248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)