FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449543
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAA18560.41286373049964076No Hit
CTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT18480.4110841454543837No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA18090.40240866835875544No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT18080.4021862202280983No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT16370.36414758988572843No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT15640.34790887634775763No Hit
TGATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG15370.341902776820015No Hit
GACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGA14930.3321150590711011No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG14880.3310028184178154No Hit
ATTTTGTGGTCTATATGCTGGAGGAGTGCGAATCCACACTCCAAAAGACA13390.2978580469499025No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG13140.2922968436834741No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA12110.26938468622578926No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT11420.2540357652104471No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT11330.25203373203453283No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11180.24869701007467584No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAAACC10430.2320134002753908No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10150.22578485261699105No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA10090.22445016383304822No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG9680.21532979047610576No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT9530.21199306851624874No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9490.21110327599362017No Hit
CTCTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC9440.20999103534033453No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9240.20554207272719185No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9090.20220535076733484No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9090.20220535076733484No Hit
ATGCAAAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG8830.19642169937024936No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT8610.1915278404957924No Hit
TGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA8280.18418705218410697No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT8090.1799605377016214No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC8080.1797380895709643No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT8020.17840340078702147No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC7980.17751360826439294No Hit
CTGTGTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT7410.1648340648169363No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7390.16438916855562205No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA7350.1634993760329935No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7320.1628320316410221No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7070.15727082837459375No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC7040.15660348398262236No Hit
CGTGTGGACTCTCTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAA7000.1557136914599938No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6890.15326676202276535No Hit
CTCCCTCTTTTCCTGACATTCATTTGCAGGAGGACATTGTTGATAGATGT6760.1503749363242226No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG6730.1497075919322512No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6590.14659331810305132No Hit
GTAACATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTT6520.14503618118845138No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6520.14503618118845138No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT6500.1445912849271371No Hit
GTGCTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6480.14414638866582286No Hit
TTGTAGGTCTTTTGGGGTTTGCCGCCCCTTTCACGCAATGTGGATATCCT6420.14281169988188003No Hit
GACCTACAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGA6410.1425892517512229No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA6380.1419219073592515No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6370.1416994592285944No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC6370.1416994592285944No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6360.14147701109793726No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6270.139474977922023No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6250.13903008166070877No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA6190.13769539287676597No Hit
AGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5920.13168929334902332No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5890.13102194895705194No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCCT5870.1305770526957377No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5850.1301321564344234No Hit
ATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA5840.12990970830376625No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA5760.1281301232585092No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5720.12724033073588067No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5710.12701788260522354No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5590.12434850503733792No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5590.12434850503733792No Hit
AGTTCATCATTACTTCCAGACGCGACATTATTTACACACTCTTTGGAAGG5570.12390360877602366No Hit
TGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGA5560.12368116064536652No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATGAACTACA5510.12256891999208085No Hit
CTCTTTGGAAGGCGGGAATCTTATATAAAAGAGAGTCCACACGTAGCGCC5510.12256891999208085No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGT5470.12167912746945231No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATGC5400.12012199055485237No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5350.11900974990156671No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5340.11878730177090958No Hit
CCTCCAGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5310.11811995737893817No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT5220.11611792420302397No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA5180.11522813168039542No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC5160.11478323541908116No Hit
GTTGTGAGTAGGCCCTGAGCCAACTGATGATCGGGGAAGAATCCCAGGGG5090.11322609850448122No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5020.11166896158988128No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT5000.11122406532856702No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC4930.10966692841396708No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4910.10922203215265282No Hit
ATTTGGTGGTCTATATGCTGGAGGAGTGCGAATCCACACTCCAAAAGACA4880.1085546877606814No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC4880.1085546877606814No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAA4860.10810979149936714No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4860.10810979149936714No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4840.10766489523805288No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4820.10721999897673859No Hit
TTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAC4810.10699755084608147No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAGGGAGTTT4780.10633020645411007No Hit
CATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGCTT4770.10610775832345294No Hit
TGATGAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG4770.10610775832345294No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4760.1058853101927958No Hit
ACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4710.10477306953951013No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4700.104550621408853No Hit
GGACATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATG4690.10432817327819587No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG4670.10388327701688158No Hit
CTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTG4560.10143634757965311No Hit
GAGCAGTAGCTGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGAT4510.10032410692636744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)