FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512930
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA19500.3801688339539508No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGT17770.34644103483906186No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16550.3226561129198916No Hit
TCCAAAGAGTGTGTAAATAATGTCTGGTCTGGAAGTAATGATTAACTACA15210.2965316904840816No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG14460.28190981225508355No Hit
CCTCCTGCATATAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGA13810.26923751778995186No Hit
GGATAATAAGGTTTAATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10970.21386933889614568No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10500.20470629520597355No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT9970.19437350125748154No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9670.18852474996588228No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGA9560.18638020782562922No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9560.18638020782562922No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACAGAAT9280.18092137328680327No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9270.18072641491041663No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9260.18053145653403No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT9000.1754625387479773No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8500.16571461992864522No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG8330.16240032753007233No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8090.15772132649679294No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7990.15577174273292652No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7910.1542120757218334No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGTCTGGA7870.15343224221628682No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7730.15070282494687384No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7610.14836332443023414No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTG7500.1462187822899811No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7420.14465911527888795No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC7380.1438792817733414No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7250.14134482288031505No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7070.13783557210535552No Hit
GCTTTAATCCTACATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG7050.1374456553525822No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACTT7040.13725069697619557No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6960.13569102996510246No Hit
GTGTAAATAATGTCTGGTCTGGAAGTAATGATTAACTACATGCTCTGGAT6840.13335152944846276No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6760.13179186243736962No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6750.13159690406098298No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6750.13159690406098298No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6740.13140194568459634No Hit
CCCCTAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCA6720.13101202893182307No Hit
CCTAAACATTAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG6550.12769773653325014No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6320.1232136938763574No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6310.12301873549997076No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA6240.12165402686526426No Hit
GTATACATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6210.12106915173610434No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6200.12087419335971769No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTACATGGCAAACTCCTT6050.11794981771391809No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5950.11600023395005167No Hit
TTCTACGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCT5920.11541535882089174No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACCAGACATTATT5900.11502544206811846No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC5890.11483048369173182No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5870.11444056693895853No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5860.1142456085625719No Hit
ACTACATGCTCTGGATAATAAGGTTTAATCCCTTTATCTAAGGGCAAATA5850.11405065018618525No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5850.11405065018618525No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTC5820.11346577505702532No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5790.1128808999278654No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5780.11268594155147875No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCACT5770.11249098317509212No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5740.11190610804593219No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5740.11190610804593219No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5660.11034644103483907No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG5630.10976156590567915No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5630.10976156590567915No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5610.10937164915290586No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5530.10781198214181273No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5530.10781198214181273No Hit
GTCGGTACACCAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGG5530.10781198214181273No Hit
CTTCAGCGACGCGGCGATTGAGACTTTCGTCTGCGAGGCGAGGGAGTTCT5480.10683719025987952No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5430.10586239837794631No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5410.10547248162517302No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGT5330.10391281461407989No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA5300.10332793948491997No Hit
GCTCTGGATAATAAGGTTTAATCCCTTTATCTAAGGGCAAATATTTAGTA5290.10313298110853333No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5260.10254810597937339No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5220.10176827247382683No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5220.10176827247382683No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5190.10118339734466691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)