FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512930
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA19200.3743200826623516No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGT17750.3460511180862886No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16850.32850486421149083No Hit
TCCAAAGAGTGTGTAAATAATGTCTGGTCTGGAAGTAATGATTAACTACA15220.2967266488604683No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG14560.28385939601894994No Hit
CCTCCTGCATATAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGA12350.24077359483750221No Hit
GGATAATAAGGTTTAATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12040.2347298851695163No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10940.21328446376698576No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10680.2082155459809331No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT10410.20295166981849377No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9770.1904743337297487No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG9590.18696508295478917No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGA9120.17780203926461702No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8840.17234320472579104No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG8490.16551966155225858No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACAGAAT8470.1651297447994853No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT8370.1631801610356189No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8200.159865868637046No Hit
GTATACATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC8130.1585011600023395No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8100.15791628487317957No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8090.15772132649679294No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8060.15713645136763302No Hit
GTGTAAATAATGTCTGGTCTGGAAGTAATGATTAACTACATGCTCTGGAT8030.15655157623847307No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7820.15245745033435362No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTG7760.15128770007603376No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7640.14894819955939406No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7490.14602382391359445No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGTCTGGA7340.14309944826779483No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7300.14231961476224828No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7230.14095490612754177No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6940.13530111321232915No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6820.13296161269568946No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6660.1298422786735032No Hit
CCCCTAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCA6640.12945236192072992No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACCAGACATTATT6610.12886748679157No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6580.12828261166241006No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACTT6560.1278926949096368No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6430.12535823601661045No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6360.12399352738190397No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC6330.12340865225274406No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6320.1232136938763574No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6160.12009435985417113No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6150.11989940147778448No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6120.11931452634862456No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC6120.11931452634862456No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA5870.11444056693895853No Hit
GCTTTAATCCTACATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG5860.1142456085625719No Hit
CCTAAACATTAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG5810.1132708166806387No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5810.1132708166806387No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5740.11190610804593219No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCACT5700.11112627454038562No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTACATGGCAAACTCCTT5670.1105413994112257No Hit
GCTCTGGATAATAAGGTTTAATCCCTTTATCTAAGGGCAAATATTTAGTA5630.10976156590567915No Hit
CTTCAGCGACGCGGCGATTGAGACTTTCGTCTGCGAGGCGAGGGAGTTCT5620.10956660752929251No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5620.10956660752929251No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5610.10937164915290586No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5610.10937164915290586No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5610.10937164915290586No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5580.10878677402374594No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5550.10820189889458601No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATTAAAC5530.10781198214181273No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5510.10742206538903944No Hit
TTCTACGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCT5490.10703214863626617No Hit
GTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTC5440.10605735675433296No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5440.10605735675433296No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5380.1048876064960131No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCATCAAATGCC5370.10469264811962646No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACCAGACATT5280.10293802273214668No Hit
CTGCCTCTTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGG5230.10196323085021347No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5200.10137835572105357No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5160.100598522215507No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5140.1002086054627337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)