FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532221
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG41650.7825696468196481No Hit
CTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT32100.6031329090734864No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG20770.3902514181139038No Hit
CTCTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGT19880.37352904150719346No Hit
GGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT18630.35004255750900476No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAA17980.33782958582994654No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT15610.29329921216938076No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCATAT14610.27451002497082977No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT13980.2626728370357427No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12680.23824689367762641No Hit
ATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTTA12210.2294159756943074No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT11920.22396711140672765No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11380.21382095031951012No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTTAGTACAATA11330.2128814909595826No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTCACATTCATTTACAGG10990.20649316731207526No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10930.2053658160801622No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT10640.1999169517925824No Hit
GTTCAGGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA10610.19935327617662588No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC10010.18807976385749528No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC9740.18300668331388653No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9710.18244300769793No Hit
ATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGAT8810.16553273921923412No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT8750.16440538798732107No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8630.16215068552349496No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8510.15989598305966882No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8510.15989598305966882No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8490.1595201993156978No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT8480.1593323074437123No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8210.15425922690010355No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA8040.15106506507634984No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7910.14862247074053822No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTCAC7830.14711933576465414No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTC7500.14091890398913232No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTTAGTACAAT7390.13885209339729174No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7330.13772474216537867No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATCTCC7280.1367852828054511No Hit
AATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTT7250.13622160718949458No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACAT6940.13039695915794378No Hit
GATTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGT6930.13020906728595827No Hit
GATTAACTGCATGTTCAGGATAATATGGTTTAATGCCTTTATCCAAGGGC6890.12945749979801624No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6760.12701490546220462No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6640.1247602029983785No Hit
CTTTTACACAATGTGGCTATCCTGCTTTAATGCCTTTATATGCATGTATA6630.124572311126393No Hit
ATATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAA6620.12438441925440746No Hit
ATTTTGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6580.12363285176646543No Hit
CATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6420.12062658181469726No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6400.12025079807072626No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAA6350.11931133871079871No Hit
ATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTTAGTACAATATGTT6280.11799609560690014No Hit
TGGGCATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTT6170.11592928501505953No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTTAGT6110.11480193378314647No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG6060.11386247442321892No Hit
TCCACAAAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6040.1134866906792479No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6020.1131109069352769No Hit
ATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGC6010.11292301506329139No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5780.10860150200762465No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG5540.10409209707997241No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5470.10277685397607386No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5380.10108582712820426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)