FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498871
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGGTGACATTGCTGAAAGTCCAA20470.41032651727600916No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT17040.34157126792297005No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG14170.28404136540308017No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG13800.2766246183883208No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12990.26038795600465847No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12900.25858388240647384No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT12450.2495635144155503No Hit
AGTCTAGACTCTGTGGTATTATGAGGATTTTTGTCAACAAGAAAAACCCC12400.24856125130544768No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12310.24675717770726302No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA11530.23112187318966226No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11270.22591010501712866No Hit
GTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTA11200.22450693666298505No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11180.22410603141894397No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCCGC11060.2217005999546977No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC10590.21227932671973315No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10580.21207887409771262No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTGATTATGCCTGCTAGG10570.21187842147569214No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10560.2116779688536716No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10560.2116779688536716No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG10470.2098738952554869No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10260.20566439019305593No Hit
GGAACATATTGTGCAGAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA10260.20566439019305593No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG9740.19524085384798875No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA9520.19083089616353727No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC8560.1715874444495671No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT8540.17118653920552607No Hit
GTACAGCAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG8450.16938246560734138No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT8400.16838020249723878No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8330.16697703414309512No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8310.16657612889905407No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC8300.16637567627703354No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8300.16637567627703354No Hit
GTATTTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG8290.16617522365501303No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG8270.16577431841097198No Hit
GTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8230.1649725079228899No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8190.16417069743480778No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8140.16316843432470518No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7950.15935983450631527No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7940.15915938188429474No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTCTGCACAATATGTT7730.15494987682186376No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGGTGACATTGC7720.15474942419984325No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7660.15354670846772012No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7450.14933720340528914No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7280.14592950883094025No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7210.1445263404767966No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7050.14131909852446825No Hit
GTTAATGAACCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGCAT7000.14031683541436565No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAAGTAGAGGACAAACGGGCAAC6920.13871321443820145No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6790.13610733035193467No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTCTGC6750.13530551986385256No Hit
ATTCTGTGTTGGGGTGAGTTAATGAACCTAGCCACCTGGGTGGGAAGTAA6720.134704161997791No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6700.13430325675374996No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6650.13330099364364736No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6610.13249918315556528No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6610.13249918315556528No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6600.13229873053354477No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6520.13069510955738056No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6510.13049465693536005No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6460.12949239382525743No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6440.12909148858121638No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA6430.12889103595919588No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6370.12768832022707272No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6340.12708696236101116No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6340.12708696236101116No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6260.12548334138484699No Hit
GTGTTGGGGTGAGTTAATGAACCTAGCCACCTGGGTGGGAAGTAATTTGG6250.12528288876282645No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6230.12488198351878542No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6230.12488198351878542No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6210.12448107827474438No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6190.12408017303070333No Hit
TCTATCAACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAAGGAGTTT6140.1230779099206007No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG6090.12207564681049811No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5960.11946976272423132No Hit
GGGTAAAGGTTCAAATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5940.11906885748019028No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5870.11766568912604661No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5850.11726478388200556No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5840.11706433125998504No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACGTTGACATAGCTGA5830.11686387863796453No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTGAATGAAAACAG5760.11546071028382086No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5740.11505980503977982No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTGCAGAAA5720.11465889979573878No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5720.11465889979573878No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5710.11445844717371827No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5600.11225346833149252No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5580.11185256308745148No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCATCAACAACC5530.11085029997734885No Hit
GGATAAAACCTAGCAGGCATAATCAATTTTAGTCTCCTGTTTTCATTCAC5520.11064984735532832No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5510.11044939473330781No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5480.10984803686724623No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC5400.10824441589108207No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5390.10804396326906154No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACTT5380.10784351064704102No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5330.1068412475369384No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA5320.10664079491491789No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5300.10623988967087684No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAATCCCC5290.10603943704885631No Hit
TAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5290.10603943704885631No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5270.10563853180481526No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5270.10563853180481526No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5260.10543807918279476No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGT5250.10523762656077422No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5250.10523762656077422No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5250.10523762656077422No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5170.10363400558461006No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC5140.10303264771854848No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCGG5120.10263174247450743No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT5100.10223083723046639No Hit
GTATGGATCGGCAGAGGAGACACGAAGGTTCCCCGCATGCGCTGATGGCC5090.10203038460844588No Hit
CTGCTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5090.10203038460844588No Hit
GCTTACATCTATCAACAATGTCCTCCCGCAAATGAATGTCAGGAAAAGAA5080.10182993198642534No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5030.10082766887632275No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5010.1004267636322817No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTGAATGAAAACAGGAGACTA5000.10022631101026117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)