FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737476
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68380.9272166145067772No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT38100.5166269817594064No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC28110.3811649463846959No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA27050.3667915972858778No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG22880.3102473843216593No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA21960.2977724020849492No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA17860.24217737255178476No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAGCATT17170.23282113587425218No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT16620.22536326605882764No Hit
GTTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAG16330.22143093470160385No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT15790.21410866251918703No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT15400.20882035483188607No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT15300.20746437850180888No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT14860.20149808264946925No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA14390.1951249938981065No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC13960.18929429567877465No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA13560.18387039035846592No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA13470.18265001166139644No Hit
GATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGAGACTA13170.17858208267116488No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA12380.16786986966355516No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11920.16163237854520013No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11870.16095439038016154No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG11800.1600052069491075No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT11710.15878482825203805No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11560.15675086375692227No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT11530.1563440708578991No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT11310.15336092293172932No Hit
ATTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11300.1532253252987216No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA11240.15241173950067527No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT10620.14400468625419674No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC10560.14319110045615044No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA10500.14237751465810414No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG10390.14088594069501922No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGAAGCTGGATCTTC10120.13722480460381084No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10030.13600442590674136No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA9870.13383486377861786No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG9260.12556340816514708No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC9150.12407183420206216No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9130.12380063893604673No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC9120.12366504130303903No Hit
TGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGA8980.12176667444093096No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8920.12095308864288465No Hit
GTTTTATCCCAATGCTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC8910.12081749100987693No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA8900.1206818933768692No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8850.12000390521183062No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT8840.11986830757882291No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG8730.118376733615738No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG8730.118376733615738No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGT8640.11715635491866853No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC8330.11295282829542927No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC8240.11173244959835982No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA8210.11132565669933665No Hit
ACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTA8190.1110544614333212No Hit
GTAACATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTT8130.11024087563527492No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8000.10847810640617457No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT7990.10834250877316685No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT7860.10657973954406653No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA7850.1064441419110588No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7780.10549495848000477No Hit
CAATGCTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATC7780.10549495848000477No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7740.10495256794797389No Hit
GGACATTGTTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATG7730.10481697031496619No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC7710.10454577504895074No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT7570.10264740818684269No Hit
CGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA7530.10210501765481182No Hit
TTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTT7400.10034224842571147No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7390.10020665079270377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)