FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463262
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGATCAGG25140.5426734763481572No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT23470.5066247609344172No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT18640.40236410497731306No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG17150.37020087984768874No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTT15310.3304825347211729No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA14240.30738545358781855No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13420.2896848867379582No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12100.2611912913211099No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT11120.24003695532981337No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10790.23291355647560127No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG10460.22579015762138918No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10180.2197460616238759No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC10130.2186667587671771No Hit
GTTAACATGGGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCCTG9900.21370196562636262No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9430.20355651877339392No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9250.19967102848927823No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9090.19621725934784204No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8920.1925476296350661No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8860.19125246620702757No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8740.18866213935095044No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8700.18779869706559138No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8640.18650353363755282No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8500.18348148563879618No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8280.17873255306932145No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8120.1752787839278853No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGATCA7780.16793952450233346No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7750.1672919427883142No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7290.1573623565066852No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAATA7240.1562830536499864No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7230.15606719307864664No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7220.15585133250730687No Hit
GTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAACCTAGCAGGCATAA7120.15369272679390927No Hit
GTATTATCCTGATCATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7100.15326100565122977No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7060.1523975633658707No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6870.14829621251041528No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6830.14743277022505621No Hit
GATCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTA6680.14419486165495982No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6670.14397900108362008No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6660.1437631405122803No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAAC6560.14160453479888271No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6380.13771904451476702No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGT6340.13685560222940796No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC6340.13685560222940796No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6050.13059564566055495No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG5750.12411982852036213No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAAT5700.12304052566366333No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5660.12217708337830428No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5660.12217708337830428No Hit
CCCTTAGACAAAGGCATTAAACCGTATTATCCTGATCATGCAGTTAATCA5640.12174536223562475No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5620.12131364109294525No Hit
GTTTTGAAGTAATGATTAACTGCATGATCAGGATAATACGGTTTAATGCC5590.12066605937892595No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5570.12023433823624644No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5420.11699642966615004No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAA5340.11526954509543194No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5250.11332679995337412No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5180.11181577595399579No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC5170.11159991538265604No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5140.11095233366863674No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5130.11073647309729699No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5060.10922544909791868No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCTCGCTTACAGACCACCAAATGCC5010.10814614624121988No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5000.10793028566988011No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4950.10685098281318131No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA4930.10641926167050178No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4870.10512409824246323No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4860.10490823767112346No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4850.10469237709978371No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG4730.10210205024370658No Hit
GGCCTAAAGATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTT4660.10059102624432827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)