FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331193
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11160.33696364355526837No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT10930.3300190523350433No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9360.2826146687882896No Hit
CTCCAAAAGAGACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9310.2811049750447624No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9200.2777836488090026No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8560.25845956889185456No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8130.24547620269752077No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7120.21498038907827158No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7020.21196100159121722No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6880.20773385910934108No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6540.1974679416533562No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6320.19082528918183658No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6290.18991947293572026No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA5980.1805593717258517No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5870.1772380454900919No Hit
CCCCCTGCATATAGACCGCCAAATGCCCCTATCTTATCAACACTTCCGGA5770.1742186580030375No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5540.16727406678281245No Hit
GTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTT5520.16667018928540156No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5410.16334886304964175No Hit
GTATACATCCTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5280.15942365931647107No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5230.15791396557294388No Hit
GCCTAGCAGCCATGGGAAGGATGTATACTTGCGGGAGAGCACGACAGAAT5180.15640427182941669No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5130.1548945780858895No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5120.15459263933718406No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5100.1539887618397732No Hit
CCCCCTGCATATAGACCGCAAAATGCCCCTATCTTATCAACACTTCCGGA5010.15127131310142425No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4990.15066743560401338No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4850.14644029312213724No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4810.14523253812731549No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4760.14372284438378832No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA4750.14342090563508286No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4740.14311896688637743No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4700.14191121189155567No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4610.13919376315320672No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4430.13375886567650888No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4390.1325511106816871No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4390.1325511106816871No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA4340.13104141693815993No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4300.12983366194333817No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACA4200.1268142744562838No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4180.12621039695887293No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4170.1259084582101675No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4150.1253045807127566No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC4120.12439876446664029No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA4100.12379488696922943No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4060.12258713197440768No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4030.12168131572829137No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4020.12137937697958591No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC3960.1195677444873533No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3940.11896386698994242No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3920.11835998949253156No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3880.1171522344977098No Hit
CCAAAAGAGACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA3880.1171522344977098No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3810.11503866325677174No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3770.11383090826194998No Hit
TGATCAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGG3730.11262315326712824No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT3720.1123212145184228No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT3680.11111345952360104No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC3670.1108115207748956No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA3640.10990570452877929No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT3620.10930182703136843No Hit
GTCTATATGCAGGGGGAGTGCGAATCCACACTCCAAAAGAGACCAAATAT3620.10930182703136843No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA3580.10809407203654665No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAC3570.10779213328784122No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3570.10779213328784122No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3550.10718825579043036No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC3490.10537662329819773No Hit
GTCGGTACACCAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGG3460.10447080705208141No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA3450.10416886830337597No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA3450.10416886830337597No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG3440.10386692955467054No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3430.1035649908059651No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTATG3410.10296111330855423No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTA3400.1026591745598488No Hit
CCGCCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3370.10175335831373249No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG3370.10175335831373249No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3370.10175335831373249No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT3360.10145141956502703No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3360.10145141956502703No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC3320.10024366457020528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)