FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331193
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT10730.3239802773609346No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9920.2995232387157941No Hit
CTCCAAAAGAGACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9720.29348446374168535No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9450.28533211752663856No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA8560.25845956889185456No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8560.25845956889185456No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7930.239437427723412No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6900.20833773660675195No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6760.20411059412487584No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6520.19686406415594535No Hit
GTATACATCCTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6450.19475049291500726No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6270.1893155954383094No Hit
CCCCCTGCATATAGACCGCCAAATGCCCCTATCTTATCAACACTTCCGGA6200.18720202419737134No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6150.18569233045384412No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6090.18388069796161152No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5990.18086131047455714No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA5890.17784192298750276No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT5740.1733128417569212No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5440.16425467929575807No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5280.15942365931647107No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA5230.15791396557294388No Hit
CCCCCTGCATATAGACCGCAAAATGCCCCTATCTTATCAACACTTCCGGA5190.15670621057812212No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5160.1558003943320058No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5020.15157325185012968No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4960.14976161935789706No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4940.14915774186048616No Hit
GCCTAGCAGCCATGGGAAGGATGTATACTTGCGGGAGAGCACGACAGAAT4910.14825192561436987No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4790.14462866062990462No Hit
GTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTT4750.14342090563508286No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4720.14251508938896654No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA4720.14251508938896654No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4630.13979764065061762No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4430.13375886567650888No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4400.13285304943039256No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACA4300.12983366194333817No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4300.12983366194333817No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4250.12832396819981098No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC4240.12802202945110555No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4240.12802202945110555No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4200.1268142744562838No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4200.1268142744562838No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4160.12560651946146204No Hit
GTCTATATGCAGGGGGAGTGCGAATCCACACTCCAAAAGAGACCAAATAT4140.12500264196405117No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4100.12379488696922943No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4070.12288907072311311No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4060.12258713197440768No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4050.12228519322570224No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA4050.12228519322570224No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4010.12107743823088048No Hit
ATTTTGCGGTCTATATGCAGGGGGAGTGCGAATCCACACTCCAAAAGAGA3970.11986968323605873No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA3970.11986968323605873No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3920.11835998949253156No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3880.1171522344977098No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG3710.11201927576971736No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3660.11050958202619017No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA3590.10839601078525211No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3590.10839601078525211No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTATG3550.10718825579043036No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA3530.10658437829301948No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC3500.10567856204690317No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTGT3480.1050746845494923No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG3480.1050746845494923No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA3480.1050746845494923No Hit
GTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT3470.10477274580078684No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC3420.10326305205725966No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC3410.10296111330855423No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA3390.10235723581114335No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTGTTTGCTG3390.10235723581114335No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT3350.1011494808163216No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT3350.1011494808163216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)