FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503283
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA31750.6308577877655315No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCCGG23490.4667354152633806No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT22170.44050762692163253No Hit
CATTTCTTGAGTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACT21860.43434807056864627No Hit
ATCCTAACCTTACCAAATATTTACCCTTAGATAAAGGCATTAAACCTTAT17630.35029993065531717No Hit
TAGACAAAGGCATTAAACCTTATTATCCGGAACATGTAGTTAATCATTAC17560.34890906309173964No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15970.31731649986190674No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15890.3157269369321038No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT15220.3024143473950044No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13450.2672452675731149No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA12980.2579065853605228No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGA12560.24956137997905753No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA11890.23624879044195807No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA11350.22551924066578846No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11140.22134663797505577No Hit
GGTTAGGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10720.2130014325935905No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10220.20306666428232226No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACA10150.20167579671874475No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC9930.1973044986617867No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG9820.1951188496333077No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCCTGC9420.18717103498429313No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG9000.17882582960282783No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8780.17445453154586982No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8770.17425583617964446No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCCTGCAAATGAATG8720.17326235934851764No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCC8510.16908975665778497No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8340.1657119354319538No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8300.16491715396705234No Hit
CAAGCAATGTTTTCGAAAACTTCCTGTAAATAGACCTATTGATTGGAAAG8250.1639236771359255No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCA8190.16273150493857333No Hit
ATTCTATCCTAACCTTACCAAATATTTACCCTTAGATAAAGGCATTAAAC8160.16213541883989724No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC7920.1573667300504885No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7670.15239934589485438No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA7620.15140586906372758No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7570.15041239223260075No Hit
ACTGACAACAGTGCCATCAGCACCTCCTCCTGCCACCACCAATCGGCAGT7550.15001500150015No Hit
CTCCAAAAGACACCAAATACTCGAGGACAGTTTCTCTTCCAAAAGTAAGA7440.147829352471671No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATCGCTGGGAGTCCAA7390.14683587564054418No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTTCGAAAAC7090.14087501465378324No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6980.13868936562530426No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6910.13729849806172673No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG6820.13551023976569843No Hit
ACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACA6790.13491415366702234No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAATGCCTTGTAAGTTGGCGA6580.13074155097628967No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6460.1283572065815853No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6420.12756242511668386No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTTAT6350.1261715575531063No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC6330.12577416682065556No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6290.12497938535575412No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6180.12279373632727512No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA6050.12021069656634538No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6030.11981330583389464No Hit
CCCTTAGATAAAGGCATTAAACCTTATTATCCGGAACATGTAGTTAATCA5990.11901852436899318No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCGAGGA5900.11723026607296493No Hit
GTTGTCAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5830.11583939850938736No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG5800.11524331241071126No Hit
ACATATTGTTGACATCTATTGATAATATCCTCCTGCAAATGAATGTGAGG5780.11484592167826055No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5740.11405114021335909No Hit
GTTAAGGGAGTAACCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATAC5710.11345505411468298No Hit
ATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAG5650.1122628819173308No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5640.11206418655110545No Hit
ATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA5580.11087201435375327No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5510.1094811467901757No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA5480.1088850606914996No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5480.1088850606914996No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5350.10630202093056988No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5290.10510984873321769No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5160.10252680897228794No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC5120.10173202750738651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)