FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567714
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83051.4628844805659187No Hit
AAAATTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGC62681.104077052882261No Hit
AAAGCTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGC59771.052818848927453No Hit
ATAATATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAG57521.013186217003632No Hit
CTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTT40020.7049324131516926No Hit
ATTAATTTTAGTCTCCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTG35760.6298946300425919No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA23490.41376467728468913No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT22140.3899850981303966No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA21800.38399616708413037No Hit
ATTAGCTTTAGTCTCCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTG20310.3577505574990224No Hit
ATATCGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAGTCC18150.31970323085215446No Hit
TCCTAACATTCATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTG17870.3147711699905234No Hit
CTTTTATATAATATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT17700.31177670446739025No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTG15740.27725227843597305No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT14330.25241582909704535No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA14240.2508305238200925No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14130.2488929284815946No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT13140.23145457043511347No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13120.23110228037356836No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12340.2173629679733105No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC11450.2016860602345547No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTG11240.19798701458833146No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10980.1934072437882455No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10680.1881228928650694No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10190.17949178635721508No Hit
TTAGCTTTAGTCTCCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTGC10160.17896335126489746No Hit
CCCCAGAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT10030.1766734658648545No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC9910.17455972549558404No Hit
GCACCGGACCATGCAAAACCTGCACAACGCCTGCTCAAGGAACCTCTATG9860.17367900034172135No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9210.16222957334150648No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9170.16152499321841632No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT8950.1576498025414205No Hit
GTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTT8890.15659293235678529No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT8610.15166087149515425No Hit
AATGCGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCTGTCT8580.15113243640283663No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8390.14778568081815843No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT8270.14567194044888798No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGT8260.14549579541811547No Hit
TGATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG8080.1423251848642098No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA8050.1417967497718922No Hit
CATCAATATAACTGAAAGCCAGACAATGGGGGAAAGCCCTACGAACCACT7870.13862613921798653No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7800.13739312400257875No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7770.13686468891026116No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7720.13598396375639848No Hit
CTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGG7460.1314041929563125No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7340.12929045258704205No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT7300.12858587246395192No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG7130.12559140694081877No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7080.12471068178695612No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7010.12347766657154835No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT6970.1227730864484582No Hit
CTTCTGGGGTTTGCCGCCCCTTTCACGCAATGTGGATATCCTGCTTTAAT6960.12259694141768566No Hit
ATATAATATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA6930.12206850632536805No Hit
AATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATTGGGA6920.12189236129459553No Hit
GCCTAGCAGCCATGGAAATGAAGTATACTTGCGGGAGAGCACGACAGAAT6820.12013091098687013No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT6790.11960247589455253No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6570.11572728521755674No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT6560.11555114018678418No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6520.11484656006369404No Hit
CTTTTTTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGG6330.11149980447901583No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6160.10850533895588271No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC6110.10762461380202004No Hit
GGAAAGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT6010.10586316349429467No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5990.10551087343274959No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5930.10445400324811437No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5910.1041017131865693No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5870.10339713306347915No Hit
TCCTCAGGCCATGCGGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5780.10181182778652631No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5730.10093110263266364No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5700.10040266754034602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)