FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567714
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGC62811.106366938282304No Hit
ATAATATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAG56700.9987423244802842No Hit
AAAGCTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGC56250.99081579809552No Hit
CTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTT41850.7371669537830668No Hit
ATTAATTTTAGTCTCCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTG33750.594489478857312No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA23230.4091849064846032No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT22580.39773547948438825No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA21790.38382002205335786No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19290.3397837643602237No Hit
CTTTTATATAATATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT18930.3334425432524123No Hit
ATTAGCTTTAGTCTCCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTG18610.32780590226769113No Hit
ATATCGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAGTCC18080.3184702156367467No Hit
TCCTAACATTCATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTG17130.3017364377133557No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTG16780.29557136163631686No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15690.2763715532821104No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15200.267740446774256No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA14200.25012594369700236No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT13370.23550590614288178No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12330.21718682294253797No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTG12090.21295934220399707No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC11740.2067942661269583No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA11480.20221449532687236No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10980.1934072437882455No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT10640.18741831274197923No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10500.18495228231116373No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10240.18037251151107775No Hit
GCACCGGACCATGCAAAACCTGCACAACGCCTGCTCAAGGAACCTCTATG10240.18037251151107775No Hit
TTAGCTTTAGTCTCCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTGC10190.17949178635721508No Hit
CCCCAGAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT10060.1772019009571721No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT9940.17508816058790166No Hit
GTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTT9800.17262213015708613No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC9210.16222957334150648No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9070.15976354291069095No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8970.15800209260296558No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG8560.15078014634129158No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT8440.1486664059720211No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8380.1476095357873859No Hit
TGATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG8350.1470811006950683No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGT8170.1439104901411626No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8160.1437343451103901No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG8130.14320591001807248No Hit
CTTCTGGGGTTTGCCGCCCCTTTCACGCAATGTGGATATCCTGCTTTAAT7910.13933071934107666No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7650.1347509485409907No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA7630.13439865847944563No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7610.13404636841790057No Hit
CTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGG7460.1314041929563125No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG7170.12629598706390893No Hit
CATCAATATAACTGAAAGCCAGACAATGGGGGAAAGCCCTACGAACCACT7140.12576755197159134No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7100.12506297184850118No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7000.12330152154077581No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA7000.12330152154077581No Hit
AATGCGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCTGTCT6950.12242079638691314No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT6900.12154007123305044No Hit
ATATAATATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA6880.12118778117150537No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6880.12118778117150537No Hit
GCCTAGCAGCCATGGAAATGAAGTATACTTGCGGGAGAGCACGACAGAAT6840.12048320104841523No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT6760.11907404080223492No Hit
GGAAAGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT6710.11819331564837225No Hit
CTTTTTTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGG6690.11784102558682716No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6660.11731259049450955No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6520.11484656006369404No Hit
AATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATTGGGA6400.1127328196944236No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT6380.11238052963287853No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA6150.10832919392511017No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC6120.10780075883279257No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6090.10727232374047495No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5970.10515858337120453No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5960.10498243834043197No Hit
TCCTCAGGCCATGCGGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5960.10498243834043197No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5850.10304484300193406No Hit
TTCCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCAC5840.10286869797116155No Hit
ATATTGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAGTCC5830.102692552940389No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5800.10216411784807139No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5730.10093110263266364No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5710.10057881257111854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)