FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515075
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27180.5276901422122992No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGT19600.3805271077027617No Hit
TCCAAAGAGTGTGTAAATAATGTCTGGTCTGGAAGTAATGATTAACTACA15840.3075280298985585No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14330.27821191088676406No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14280.27724117846915497No Hit
GGATAATAAGGCTTAATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13900.2698636120953259No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA13390.2599621414357132No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12890.2502548172596224No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG10210.19822355967577537No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT10070.19550550890646995No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9820.1906518468184245No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGA9790.19006940736785904No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTATCTCTTCCAAAAGTAAGA9560.18560403824685726No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9470.1838567198951609No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT9140.17744988593894093No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA8930.1733728097849828No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG8890.17259622385089549No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT8660.16813085472989373No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG8640.16774256176285007No Hit
ACGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCC8380.16269475319128282No Hit
CGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCT8170.15861767703732466No Hit
GTGTAAATAATGTCTGGTCTGGAAGTAATGATTAACTACATGCTCTGGAT7800.15143425714701741No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACCAGACATTATT7570.14696888802601563No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7450.14463913022375383No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7140.13862058923457748No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG7140.13862058923457748No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTG6750.13104887637722662No Hit
GCACCGGACCATGCAAAACCTGCACAGCTCCTGCTCAAGGAACCTCTATG6690.1298839974760957No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTGGTCTGGA6600.12813667912439936No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6460.12541862835509393No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6430.12483618890452845No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATTAAGC6420.12464204242100664No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6340.12308887055283212No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG6300.12231228461874483No Hit
GCAATAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATTAGACAACT6190.12017667330000487No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6150.11940008736591758No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6140.11920594088239576No Hit
CCCTCAGAGGTGGAGATAAGGGAGTAGGCTGCCTTCCTGTCTGCCGATTG6090.11823520846478669No Hit
ACTACATGCTCTGGATAATAAGGCTTAATCCCTTTATCTAAGGGCAAATA6080.11804106198126486No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6070.11784691549774305No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6050.11745862253069943No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC6020.11687618308013395No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6000.11648789011309033No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTAGGGGAGGAGATTA5960.11571130417900306No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5950.11551715769548125No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5940.11532301121195943No Hit
ACACCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGG5940.11532301121195943No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5920.1149347182449158No Hit
CCCAAAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGAAC5900.11454642527787215No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5890.11435227879435034No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5740.11144008154152307No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCAACCACTTT5730.11124593505800126No Hit
ACATTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCACTCCC5680.11027520264039219No Hit
TTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5670.11008105615687036No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA5580.10833373780517401No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5540.10755715187108675No Hit
ACGTTAATATGGGCCTAAAAACTAGACAACTATTGTGGTTTCACATTTCC5510.10697471242052127No Hit
CTACCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC5460.1060039800029122No Hit
TGATCAACTACATGCTCTGGATAATAAGGCTTAATCCCTTTATCTAAGGG5450.10580983351939038No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5390.10464495461825947No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACAGAAT5380.10445080813473766No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG5270.10231519681599768No Hit
GCTCTGGATAATAAGGCTTAATCCCTTTATCTAAGGGCAAATATTTAGTA5210.10115031791486677No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATTAAGCCTTAT5180.10056787846430132No Hit
GACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTTGAGTTCCACTGCA5160.1001795854972577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)