FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454547
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCCGG12510.27521906425518156No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT11840.2604791143710111No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATCGCTGGGAGTCCAA9640.21207927893045164No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8870.1951393365262558No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8470.18633936644615406No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8220.1808393851460905No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7920.17423940758601422No Hit
TTCTACTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7640.16807942852994298No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7590.16697943226993028No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7440.16367944348989213No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7290.16037945470985399No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7200.1583994614418311No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA7070.15553947116579805No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7050.15509947266179294No Hit
CAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG7020.15443947490578533No Hit
CCCCAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6900.1517994838817548No Hit
ACAATATGTAGGCCCTCTAACAGTTAATGAAAAAAGGAGATTAAAATTAA6600.1451995063216785No Hit
ATTAATAGGTGTCAACAATATGTAGGCCCTCTAACAGTTAATGAAAAAAG6470.14233951604564543No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6370.14013952352562No Hit
CATTAAGATTCCCGAGATTGAGATCTTCGGCGACGCGGCGATTGAGACCT6160.1355195392335666No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6140.13507954072956152No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6090.1339795444695488No Hit
CTCTAACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6080.13375954521754627No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA5980.13155955269752082No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5960.13111955419351573No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC5920.13023955718550556No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA5670.12473957588544199No Hit
CAAAACACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG5640.12407957812943435No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5420.11923959458537842No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5410.11901959533337587No Hit
GGCCCATAGCCAAGCCCCAGCCAGTGGGGGTTGCATCAGCAAACACTTGG5370.11813959832536569No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC5360.11791959907336315No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG5250.11549960730133518No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGTTGGAATGTTGTGG5110.11241961777329956No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5090.11197961926929449No Hit
CCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGACA5020.11043962450527668No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4840.1064796379692309No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4830.10625963871722836No Hit
ATGTAGGCCCTCTAACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG4800.10559964096122072No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG4780.10515964245721564No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4750.10449964470120802No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4730.10405964619720294No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4680.10295964993719021No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4650.10229965218118259No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4650.10229965218118259No Hit
CTATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATC4600.10119965592116986No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC4580.10075965741716479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)