FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454547
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCCGG14890.3275788862317868No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT9600.21119928192244145No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATCGCTGGGAGTCCAA8710.1916193484942151No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8640.1900793537301973No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8420.18523937018614137No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8170.1797393888860778No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7520.16543943750591247No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7350.16169945022186924No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7260.15971945695384637No Hit
TTCTACTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7130.15685946667781328No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA6920.1522394823857599No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6760.1487194943537192No Hit
CCCCAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6630.14585950407768614No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6630.14585950407768614No Hit
CAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG6600.1451995063216785No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6290.13837952950959967No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6280.1381595302575971No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6180.1359595377375717No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6070.13353954596554374No Hit
ACAATATGTAGGCCCTCTAACAGTTAATGAAAAAAGGAGATTAAAATTAA6050.13309954746153863No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5930.13045955643750812No Hit
CTCTAACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5750.12649956990146233No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5740.1262795706494598No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA5700.1253995736414496No Hit
ATTAATAGGTGTCAACAATATGTAGGCCCTCTAACAGTTAATGAAAAAAG5610.12341958037342673No Hit
CATTAAGATTCCCGAGATTGAGATCTTCGGCGACGCGGCGATTGAGACCT5520.12143958710540384No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5520.12143958710540384No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG5440.1196795930893835No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC5360.11791959907336315No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5300.11659960356134788No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC5200.11439961104132246No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5160.11351961403331229No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5050.11109962226128431No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5020.11043962450527668No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA5000.1099996260012716No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGTTGGAATGTTGTGG4990.10977962674926905No Hit
CAAAACACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG4910.10801963273324872No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG4760.10471964395321057No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4730.10405964619720294No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG4720.10383964694520037No Hit
GGCCCATAGCCAAGCCCCAGCCAGTGGGGGTTGCATCAGCAAACACTTGG4640.10207965292918003No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4580.10075965741716479No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4550.10009965966115714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)