FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353502
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGAACCTTTACCCCGTTGCTCGCCAACGGTCAGGTCTTTGCCAAG25430.7193735820447975No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA22620.6398832255545938No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCATTAAGCTCCTCC18650.527578344676975No Hit
GTTCATATATTGTTTACACAGAAAGGTCTTGTAAGTTAGCGAGAAAGTGA15790.4466735690321412No Hit
CCATTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT14840.419799605094172No Hit
GTTCCCATATTGTTTACACAGAAAGGTCTTGTAAGTTAGCGAGAAAGTGA14040.39716889862009264No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA13640.385853545383053No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT13540.3830247070737931No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12460.352473253333786No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA10280.2908045781919197No Hit
ATATGGGAACCTTTACCCCGTTGCTCGCCAACGGTCAGGTCTTTGCCAAG9930.28090364410950996No Hit
CATTTAAACCCTACTAAAACTAAACGGTGGGGCTACTCCCTTAACTTCAT9460.2676081040559884No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC9200.26025312445191257No Hit
GTTCACATATTGTTTACACAGAAAGGTCTTGTAAGTTAGCGAGAAAGTGA8360.23649088265412926No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8320.23535934733042527No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8130.22998455454283145No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA8100.22913590305005346No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTGAAG7780.2200836204604217No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAACGCATTGTGGGGCTTCTGG7560.2138601761800499No Hit
AACGCATTGTGGGGCTTCTGGGCTTTGCCGCTCCCTTCACACAATGTGGT7540.2132944085181979No Hit
CCCTTCACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7510.21244575702541996No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC7500.21216287319449395No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7480.21159710553264197No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7100.20084751995745426No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6880.19462407567708245No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6840.19349254035337848No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGG6650.18811774756578464No Hit
ATATTAACATTCACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTC6650.18811774756578464No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6530.18472314159467274No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6470.18302583860911678No Hit
CCCAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC6220.175953742835967No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6190.17510509134318902No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6000.16973029855559515No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5780.16350685427522332No Hit
CCGATAAGTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAG5610.15869782914948147No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5610.15869782914948147No Hit
ACTTCTCGGTACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT5560.15728340999485152No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5540.15671764233299953No Hit
ATATGTGAACCTTTACCCCGTTGCTCGCCAACGGTCAGGTCTTTGCCAAG5510.15586899084022154No Hit
GTGTGAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT5440.1538888040237396No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5370.15190861720725768No Hit
CCTTTACCCCGTTGCTCGCCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5300.14992843039077572No Hit
TATGTGAACCTTTACCCCGTTGCTCGCCAACGGTCAGGTCTTTGCCAAGT5280.14936266272892373No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5260.14879689506707175No Hit
TTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCT5240.14823112740521976No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA5170.14625094058873783No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5120.14483652143410786No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5080.1437049861104039No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5010.14172479929392195No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4910.13889596098466203No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCATTAAGCTCCTCCCCC4910.13889596098466203No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4880.13804730949188407No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4750.13436981968984615No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG4740.13408693585892018No Hit
ATATGCGAACCTTTACCCCGTTGCTCGCCAACGGTCAGGTCTTTGCCAAG4720.1335211681970682No Hit
GGTCGATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT4720.1335211681970682No Hit
TGTTCATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4690.13267251670429023No Hit
GAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTTCCTGTGGT4630.13097521371873427No Hit
CTGCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGT4610.1304094460568823No Hit
ATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA4570.12927791073317832No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4540.12842925924040033No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4510.12758060774762237No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGG4470.1264490724239184No Hit
GCCTTCGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4430.12531753710021443No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTC4430.12531753710021443No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4420.12503465326928845No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4340.12277158262188051No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4330.1224886987909545No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4300.12164004729817654No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4280.12107427963632454No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4270.12079139580539855No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4270.12079139580539855No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC4260.12050851197447257No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC4250.12022562814354656No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4190.11852832515799062No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4100.1159823706796567No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4080.1154166030178047No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4040.11428506769410073No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCTAACTTACA3990.11287064853947079No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG3980.11258776470854479No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3970.11230488087761879No Hit
GTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3900.11032469406113685No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3890.11004181023021085No Hit
GTTTCCCACCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGAT3880.10975892639928488No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3850.10891027490650688No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3850.10891027490650688No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCTAACTTACAAGACC3840.1086273910755809No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGCTTA3820.10806162341372892No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3800.10749585575187694No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3780.10693008809002495No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT3750.10608143659724698No Hit
GTTTAGTTTTAGTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTG3750.10608143659724698No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG3710.104949901273543No Hit
ATTTATGCCTACAGCCTCCATTAAGCTCCTCCCCCAACTCCTCCCAGTCT3680.10410124978076504No Hit
GTTCGCATATTGTTTACACAGAAAGGTCTTGTAAGTTAGCGAGAAAGTGA3670.10381836594983904No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3650.10325259828798707No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT3650.10325259828798707No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC3620.10240394679520907No Hit
ATATGAACCTTTACCCCGTTGCTCGCCAACGGTCAGGTCTTTGCCAAGTG3610.1021210629642831No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3580.1012724114715051No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3550.10042375997872713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)