FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510097
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52231.023922900938449No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23460.4599125264410494No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA22270.43658363017230056No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15150.2970023348500383No Hit
ATTGTATGTTGGGGAGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA13700.2685763688082855No Hit
CCTCCTGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12350.24211081421768801No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG12180.23877811475072389No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11390.2232908642865965No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTTCCAATTACATATCCCATGAA11160.21878191794893914No Hit
GTTTACAGGAAGCTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT11110.21780171222336142No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9800.1921203222132261No Hit
ATTCTATGTTGGGGAGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA9780.19172823992299504No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA9570.18761137587556875No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9330.18290638839279588No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9270.18173014152210265No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9030.17702515403932978No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8810.17271224884678796No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8800.17251620770167242No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA8710.1707518373956326No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8570.1680072613640151No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8330.1633022738812422No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8230.16134186243008683No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT8090.15859728639846932No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG8030.15742103952777609No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7870.1542843812059275No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG7840.1536962577705809No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAGCTTCCTGTAA7840.1536962577705809No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7760.1521279286096566No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7640.14977543486827014No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7500.14703085883665262No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7500.14703085883665262No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7350.1440902416599196No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7310.14330607707945744No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6900.13526839012972044No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6890.1350723489846049No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6880.13487630783948934No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6860.1344842255492583No Hit
GAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGT6810.1335040198236806No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG6740.13213173180787183No Hit
ACATACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG6730.1319356906627563No Hit
CAATAGGCCTGTTTACAGGAAGCTTCCTAAAACACATTTTGATTTTTTGT6660.13056340264694755No Hit
CTCTAAGGCCTCCCGATGCAAAGCAGAGGCGGTGTCGAGAAGATCTCGAA6590.12919111463113878No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT6550.12840695005067662No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6550.12840695005067662No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6430.12605445630929019No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6400.1254663328739436No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6370.12487820943859697No Hit
CTATTCTATGTTGGGGAGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT6200.12154550997163285No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6040.11840885164978426No Hit
CTCCAAAAGACACCAAATATTCAAGAACTGTTTCTCTCCCAAAAGTAAGA5990.11742864592420657No Hit
GTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACAT5980.11723260477909103No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5880.11527219332793566No Hit
GGTCCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGG5850.11468406989258906No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5800.11370386416701136No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5750.11272365844143369No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAAC5720.11213553500608708No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5570.10919491782935403No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACATTGCCACAG5560.10899887668423848No Hit
GTTGATAGATGTAGGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5510.10801867095866081No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5490.10762658866842972No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5400.1058622183623899No Hit
CCCTTTTCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5400.1058622183623899No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5360.10507805378192775No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5320.10429388920146561No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5290.10370576576611899No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5220.10233347775031024No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5220.10233347775031024No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5190.10174535431496362No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5180.10154931316984808No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAGGCTTTTACTTT5110.10017702515403933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)