FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394523
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA25640.6498987384765907No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC25630.649645267829759No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT20970.5315279464061664No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT16740.42430986279633887No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14480.36702549661236483No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT13640.34573396227849834No Hit
CATCTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT11560.29301206773749566No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGACGTTCACGGTGGTCTCCATGC10730.2719740040504609No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG10090.25575188265322935No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9700.24586652742679135No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9510.2410505851369882No Hit
GGTTTAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG9320.2362346428471851No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9050.22939093538272803No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8990.22787011150173755No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8570.2172243443348043No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8550.21671740304114082No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT8500.21545004980698212No Hit
TTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8110.20556469458054408No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT7960.20176263487806795No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7910.2004952816439092No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT7870.19948139905658227No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7750.19643975129460134No Hit
GTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA7740.19618628064776958No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7670.19441198611994737No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC7410.18782174930232204No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7030.17818986472271578No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6950.17616209954806183No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6840.17337392243291266No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6770.17159962790509045No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6540.16576980302796035No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6360.16120733138498897No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6290.15943303685716675No Hit
GTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAG6220.15765874232934457No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6160.1561379184483541No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6060.15360321198003665No Hit
GATGTCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACT5930.150308093571224No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5920.15005462292439223No Hit
GGGTAAAGGTTTAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5900.14954768163072876No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5900.14954768163072876No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG5880.14904074033706527No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5850.14828032839657004No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAATCTAATCTCCTCC5840.14802685774973828No Hit
TTCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5800.14701297516241132No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5670.14371785675359863No Hit
GTCTTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTT5630.14270397416627167No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTG5530.14016926769795424No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5510.13966232640429077No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5480.13890191446379552No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5470.13864844381696378No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5360.1358602667018146No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5350.13560679605498285No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5330.13509985476131936No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5260.13332556023349715No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5250.13307208958666542No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGTCC5190.13155126570567496No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5120.12977697117785275No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5100.12927002988418926No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5090.12901655923735753No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5050.12800267665003054No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5020.1272422647095353No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4980.12622838212220833No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAGATGTATA4980.12622838212220833No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4970.1259749114753766No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG4940.12521449953488134No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGG4920.12470755824121789No Hit
CTCTAAGGCCTCCCGATACAGAGCGGAGGCGGTGTCGAGGAGATCACGAA4890.12394714630072265No Hit
CCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCA4820.12217285177290045No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4820.12217285177290045No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4800.12166591047923696No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4800.12166591047923696No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4720.11963814530458301No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4720.11963814530458301No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4690.11887773336408777No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4640.11761038012992905No Hit
CTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG4620.11710343883626556No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGACGTTC4590.11634302689577034No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4590.11634302689577034No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4550.11532914430844336No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4520.11456873236794812No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4520.11456873236794812No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4470.1133013791337894No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4450.11279443784012592No Hit
GGGTAACCACATTGTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAAT4420.11203402589963068No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4410.11178055525279894No Hit
TTCTATTCGTGATCTCCTCGACACCGCCTCCGCTCTGTATCGGGAGGCCT4390.11127361395913546No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4370.11076667266547197No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT4370.11076667266547197No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4250.10772502490349106No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCGAGCAGCC4250.10772502490349106No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4180.10595073037566885No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4110.10417643584784665No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4070.10316255326051967No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4060.10290908261368792No Hit
TCCCGATACAGAGCGGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA4040.10240214132002444No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC4030.10214867067319269No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4010.1016417293795292No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4000.10138825873269747No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT4000.10138825873269747No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT3970.10062784679220223No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG3960.10037437614537048No Hit
GACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAATCTAATCT3960.10037437614537048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)