FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506946
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26940.5314175474310874No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT17850.352108508598549No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17550.34619071853806915No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA16170.318968884259862No Hit
CCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTC13490.266103293052909No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA12860.2536759339259014No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTTAT11550.22783491732847283No Hit
GGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTAACATT11150.21994453058116642No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8970.17694192280834645No Hit
CCCAATGACATATCCCATAAAGTTAAGGGAATATCCCCATCTTTTTGTTT8710.17181317142259728No Hit
ATGCAGTAGGAGTGCGAATCCACACCCCAAAAGACACCAAATATTCAAGA8500.1676707183802614No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8490.16747345871157873No Hit
GTTTACAGGAAGTTTCCTAAAACACAATTTGATTTTTTGTACAATATGTT8070.159188552626907No Hit
CCCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8040.15859677362085903No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7970.1572159559400804No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7940.1566241769340324No Hit
CCCTTACAGTAAATGAAAATAGGAGACTAAAATTAATTATGCCTGCTAGG7890.15563787859061912No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7820.1542570609098405No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC7800.15386254157247517No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATACAAAGACC7650.15090364654223526No Hit
GCCTAGCAGCCATGGAAAAGAGGTATACTTGCGGGAGAGCACGACAGAAT7640.15070638687355262No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7270.14340777913229416No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACAATTTGATTTTTTGT7200.14202696145151553No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA7150.14104066310810223No Hit
GCTCAGAATACTGTCTCTACCATATCGTCAATCTTATCGAAGACTGGGGA7140.1408434034394196No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATGACATATCCCATAAA6970.13748998907181434No Hit
ACCGTCCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTCTGTGCCTT6920.13650369072840104No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6890.1359119117223531No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT6690.13196671834869986No Hit
CACCGGAACTTGCCCAAGGTCTTGCATAAGAGGACTCTTGGACTTTCAGC6400.1262461879569027No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6270.12368181226402812No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6160.12151195590851885No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6120.12072291723378822No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5920.116777723860135No Hit
ATTCGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5890.11618594485408701No Hit
GTCATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAAAT5890.11618594485408701No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5880.11598868518540437No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGAATCAAAC5830.11500238684199107No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5830.11500238684199107No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5780.11401608849857776No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5690.11224075148043382No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCCTCTGTGG5690.11224075148043382No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACACGCAAAACAG5600.11046541446228988No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5580.11007089512492456No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5580.11007089512492456No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGATTA5580.11007089512492456No Hit
AGAACATATTGTACAAAAAATCAAATTGTGTTTTAGGAAACTTCCTGTAA5550.10947911611887656No Hit
GCACCGGACCATGCAAAACCTGCACAGCTCCTGCTCAAGGAACCTCTATG5530.10908459678151125No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5480.10809829843809794No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5450.10750651943204996No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTC5410.10671748075731932No Hit
GTGGTCGGTCGGTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCG5410.10671748075731932No Hit
CCCTTAACTTTATGGGATATGTCATTGGGAGTTGGGGCACATTGCCACAA5390.106322961419954No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5360.10573118241390601No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC5360.10573118241390601No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5330.10513940340785805No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5240.1033640663897141No Hit
GTTCCAGTGGGCGTTCACGGTGGCCTCCATGCGACGTGCAGAGGTGAAGC5230.10316680672103143No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA5100.10060243102815683No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5080.10020791169079153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)