FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506946
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89291.7613315816674753No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT17690.34895235389962637No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17420.34362634284519455No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA15020.29628402236135604No Hit
GGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTAACATT11820.23316092838290467No Hit
CCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTC11070.21836645323170517No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA10820.21343496151463864No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTTAT10200.20120486205631366No Hit
GTTTACAGGAAGTTTCCTAAAACACAATTTGATTTTTTGTACAATATGTT8920.17595562446493315No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8540.16845975705499205No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC7640.15070638687355262No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7640.15070638687355262No Hit
CCCTTACAGTAAATGAAAATAGGAGACTAAAATTAATTATGCCTGCTAGG7540.148733790186726No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7530.14853653051804333No Hit
CCCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7390.1457748951564861No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT7350.14498585648175547No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATACAAAGACC7270.14340777913229416No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7190.14182970178283288No Hit
GCCTAGCAGCCATGGAAAAGAGGTATACTTGCGGGAGAGCACGACAGAAT6920.13650369072840104No Hit
GCTCAGAATACTGTCTCTACCATATCGTCAATCTTATCGAAGACTGGGGA6850.13512287304762244No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6660.13137493934265188No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA6640.13098042000528656No Hit
CCCAATGACATATCCCATAAAGTTAAGGGAATATCCCCATCTTTTTGTTT6630.1307831603366039No Hit
ATGCAGTAGGAGTGCGAATCCACACCCCAAAAGACACCAAATATTCAAGA6610.13038864099923858No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACAATTTGATTTTTTGT6560.1294023426558253No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6460.12742974596899867No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6410.12644344762558535No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATGACATATCCCATAAA6400.1262461879569027No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGAATCAAAC6210.12249825425193217No Hit
CACCGGAACTTGCCCAAGGTCTTGCATAAGAGGACTCTTGGACTTTCAGC6170.12170921557720152No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6170.12170921557720152No Hit
ACCGTCCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTCTGTGCCTT6160.12151195590851885No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6150.12131469623983619No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6020.11875032054696161No Hit
GTCATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAAAT5580.11007089512492456No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGATTA5580.11007089512492456No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5470.10790103876941529No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5420.10691474042600199No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5250.10356132605839675No Hit
CCCTTAACTTTATGGGATATGTCATTGGGAGTTGGGGCACATTGCCACAA5170.10198324870893548No Hit
CCTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGG5070.10001065202210886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)