FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332755
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19000.5709906688103861No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16320.4904509323676579No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAACCTTAT13390.4023981608090036No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9560.2872984628330153No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9410.2827906417634596No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8720.2620546648435035No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8580.2578473651785848No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7880.2368108668539917No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7810.23470721702153235No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7710.2317020029751619No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7620.2289973103334285No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA7620.2289973103334285No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7310.2196811467896801No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7040.21156706886447987No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAAC6890.20705924779492418No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCTTCCACCAATCGGCAGT6520.1959399558233535No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6510.19563943441871645No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6350.19083109194452375No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG6350.19083109194452375No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6150.18482066385178283No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6080.18271701401932353No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAAC5540.1664888581689231No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTC5450.16378416552718966No Hit
AGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTG5430.16318312271791557No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5300.159276344457634No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA5230.15717269462517466No Hit
GTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC5180.15567008760198944No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5170.1553695661973524No Hit
GGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTT5160.15506904479271535No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5110.15356643776953013No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5070.15236435215098196No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5010.1505612237231597No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4960.14905861669997444No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4930.1481570524860633No Hit
GGAACATATTGTCCAAAAACTCAAGCAATGTTTTCGGAAATTGCCTGTAA4930.1481570524860633No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4780.14364923141650765No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4760.14304818860723353No Hit
GTGTAGTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAA4710.1415455815840483No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4580.13763880332376674No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4570.1373382819191297No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4500.1352346320866704No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4380.13162837523102583No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTCCGAAAAC4350.1307268110171147No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4330.1301257682078406No Hit
GTATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT4270.12832263978001834No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4250.12772159697074423No Hit
CTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4200.126218989947559No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4190.12591846854292196No Hit
GTCTATTTACAGGCAATTTCCGAAAACATTGCTTGAGTTTTTGGACAATA4190.12591846854292196No Hit
CTCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT4170.12531742573364787No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGT4150.12471638292437379No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4130.1241153401150997No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4110.1235142973058256No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4050.12171116887800333No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4020.12080960466409219No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3980.11960751904554402No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3940.11840543342699585No Hit
GGTCTATTTACAGGCAATTTCCGAAAACATTGCTTGAGTTTTTGGACAAT3940.11840543342699585No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3930.1181049120223588No Hit
GGATAAAGGTATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT3920.11780439061772174No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG3900.11720334780844766No Hit
CTTTTACACAATGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATA3900.11720334780844766No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG3890.11690282640381061No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3870.11630178359453651No Hit
ATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCT3870.11630178359453651No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATACC3860.11600126218989948No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATCTCCTCCCC3850.11570074078526243No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACAC3820.11479917657135129No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATACCTTTATCCAAGG3820.11479917657135129No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3770.11329656954816607No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3690.1108923983110697No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3680.11059187690643266No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGTACGTTCACGGTGGTCTCCATGC3660.10999083409715857No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3630.10908926988324744No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3590.10788718426469925No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA3590.10788718426469925No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3530.10608405583687698No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3510.10548301302760289No Hit
GTTCAGGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTA3480.10458144881369176No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3420.1027783203858695No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGAGGGACGTCC3410.10247779898123245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)