FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332755
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18970.5700891045964749No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18080.5433426995837778No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAACCTTAT13100.39368304007452937No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9870.29661462637676367No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9350.2809875133356373No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8960.26926717855479254No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8680.2608525792249553No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8180.24582650899310304No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7980.23981608090036213No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7770.2335051314029842No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA7600.22839626752415443No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAAC7380.2217847966221394No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7030.21126654745984283No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6690.20104881970218327No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6530.19624047722799054No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6520.1959399558233535No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5980.17971179997295306No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5970.17941127856831604No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCTTCCACCAATCGGCAGT5910.17760815014049378No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5530.16618833676428604No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5530.16618833676428604No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTC5530.16618833676428604No Hit
GGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTT5460.1640846869318267No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAAC5280.1586753016483599No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA5270.15837478024372287No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5260.15807425883908582No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5220.15687217322053762No Hit
GTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC5210.1565716518159006No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5190.1559706090066265No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5110.15356643776953013No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5110.15356643776953013No Hit
GGAACATATTGTCCAAAAACTCAAGCAATGTTTTCGGAAATTGCCTGTAA5080.152664873555619No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG5050.15176330934170784No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGAGGGACGTCC4880.1466544454628781No Hit
AGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTG4730.14214662439332243No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4640.139441931751589No Hit
GTCTATTTACAGGCAATTTCCGAAAACATTGCTTGAGTTTTTGGACAATA4570.1373382819191297No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4550.1367372391098556No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4520.13583567489594445No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4420.132830460849574No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4350.1307268110171147No Hit
CTTTTACACAATGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATA4350.1307268110171147No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4300.12922420399392945No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTCCGAAAAC4160.12501690432901083No Hit
GTGTAGTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAA4110.1235142973058256No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4110.1235142973058256No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4100.12321377590118855No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4080.12261273309191448No Hit
CTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4070.12231221168727743No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4070.12231221168727743No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4020.12080960466409219No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4000.12020856185481811No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3970.11930699764090696No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG3960.11900647623626992No Hit
GTATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT3960.11900647623626992No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATACC3930.1181049120223588No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3910.1175038692130847No Hit
GTTCAGGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTA3900.11720334780844766No Hit
GGATAAAGGTATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT3900.11720334780844766No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGT3880.11660230499917358No Hit
ATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCT3840.11540021938062539No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG3790.11389761235744017No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3780.1135970909528031No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATACCTTTATCCAAGG3770.11329656954816607No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3760.11299604814352902No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3750.11269552673889198No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3730.11209448392961788No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3720.11179396252498083No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3700.11119291971570676No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3640.10938979128788447No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3610.10848822707397335No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA3590.10788718426469925No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3580.1075866628600622No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACAC3550.10668509864615106No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3540.10638457724151403No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT3510.10548301302760289No Hit
GGTCTATTTACAGGCAATTTCCGAAAACATTGCTTGAGTTTTTGGACAAT3510.10548301302760289No Hit
CTTTACCACAGGAACATATTGTCCAAAAACTCAAGCAATGTTTTCGGAAA3460.10398040600441767No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG3420.1027783203858695No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3400.1021772775765954No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3400.1021772775765954No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3390.10187675617195835No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA3370.10127571336268426No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3360.1009751919580472No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGTACGTTCACGGTGGTCTCCATGC3330.10007362774413608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)