FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360203
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15540.4314233918096185No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14870.41282276938282025No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13340.3703467211544601No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12370.3434174618201403No Hit
ATTTCCGAGAGAGGACAACAGAGTTGTCGGTTCCGATAAGTTTCGCCCCA8950.24847100107439415No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8760.2431961976996305No Hit
CTCGAAAATACACCTCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG8660.24041998539712328No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7930.22015363558882073No Hit
GTTTATGAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7460.2071054377670369No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7430.20627257407628477No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG7290.2023858768527747No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6980.19377961871500238No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6890.19128102764274588No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6730.18683908795873438No Hit
GTTCTATCCCAACCATACCAAATATTTGCCCTTGGACAAAGGAATTAAAC6290.17462375382770273No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6100.16934895045293905No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6090.16907132922268833No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5940.16490701076892753No Hit
GGATATCTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC5770.1601874498546653No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTAAT5760.15990982862441458No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5620.15602313140090449No Hit
GTTCTTTCCCAACCATACCAAATATTTGCCCTTGGACAAAGGAATTAAAC5530.153524540328648No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5490.15241405540764513No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT5370.1490826006446365No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5360.14880497941438578No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5280.14658400957238002No Hit
CATGTATACAAGCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5240.14547352465137714No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5220.1449182821908757No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5160.14325255480937138No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5130.1424196911186192No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5120.1421420698883685No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5090.14130920619761636No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5080.14103158496736562No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4850.13464629667159908No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4790.13298056929009475No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4770.1324253268295933No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA4720.13103722067833973No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4720.13103722067833973No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4670.1296491145270861No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA4670.1296491145270861No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4610.1279833871455818No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4590.12742814468508035No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4370.12132047761956452No Hit
GCATATTCAGGATAATACGGTTTAATTCCTTTGTCCAAGGGCAAATATTT4330.12020999269856164No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4330.12020999269856164No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4320.11993237146831093No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4240.11771140162630517No Hit
CTCATAAACTGAGCCATGAGAAACGGACTGAGGCCCACTCCCATAGGCAT4190.11632329547505157No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4190.11632329547505157No Hit
GCAGACGAAGAAGGGGACGGTAGAGTCCCAAACGGCCCCGAGATGGGTCG4170.11576805301455012No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT4150.11521281055404868No Hit
TAGGCATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGT4140.11493518932379797No Hit
GTAGAGTCCCAAACGGCCCCGAGATGGGTCGTCCGCGGGATTCAGCGCCG4080.11326946194229366No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4040.11215897702129077No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4040.11215897702129077No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4030.11188135579104005No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3980.11049324963978645No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3940.10938276471878358No Hit
GGATAATACGGTTTAATTCCTTTGTCCAAGGGCAAATATTTGGTATGGTT3920.10882752225828214No Hit
CTCGGAAATACACCTCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG3920.10882752225828214No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3900.1082722797977807No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT3880.10771703733727926No Hit
GGAAAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3840.10660655241627637No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG3840.10660655241627637No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG3790.10521844626502279No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3780.10494082503477206No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTCCGAAAAC3780.10494082503477206No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG3740.10383034011376918No Hit
GGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC3730.10355271888351845No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3730.10355271888351845No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3690.10244223396251557No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT3660.10160937027176344No Hit
GATTAACTGCATATTCAGGATAATACGGTTTAATTCCTTTGTCCAAGGGC3650.10133174904151271No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC3620.10049888535076054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)