FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294595
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG17500.5940358797671379No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC11390.38663249545986866No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8870.3010913287734008No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8790.29837573618017954No Hit
ATATATGCCCTTGGACAAAGGCATTAAACCATATTATCCTGAACATGCAG8240.2797060371017838No Hit
ATCCTAACCTTACCAAATATATGCCCTTGGACAAAGGCATTAAACCATAT8080.2742748519153414No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA7850.26646752320983047No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7810.2651097269132199No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7760.26341248154245656No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7730.26239413431999864No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7490.25424735654033503No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7430.25221066209541915No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7180.2437244352416029No Hit
GGCATATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAATTT5880.19959605560175833No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTAAGTACAATA5600.19009148152548414No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG5560.18873368522887354No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5550.1883942361547209No Hit
CTCTTATATAAAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTGGT5530.18771533800641557No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5350.18160525467166788No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG5330.18092635652336259No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5180.17583462041107284No Hit
CTTATATAAAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT5140.17447682411446225No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5110.17345847689200428No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4980.16904563892801983No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4890.16599059726064597No Hit
GAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCA4820.1636144537415774No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCATATATTTGGTAAGGTT4780.1622566574449668No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4740.16089886114835622No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4720.16021996300005092No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4700.1595410648517456No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4670.15852271762928766No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTCCTTACAGACCACCAAATGCC4670.15852271762928766No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT4460.15139428707208202No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC4430.15037593984962408No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTT4160.1412108148475025No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4140.1405319166991972No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC4120.1398530185508919No Hit
GGGTAAAGGTTCAGATATTGGTTACACAGAAAGGCCTTGTAAGTTGGCGA4030.13679797688351805No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4000.1357796296610601No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4000.1357796296610601No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA3970.13476128243860214No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG3970.13476128243860214No Hit
CTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG3870.13136679169707563No Hit
GTTCTATCCTAACCTTACCAAATATATGCCCTTGGACAAAGGCATTAAAC3870.13136679169707563No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3720.12627505558478588No Hit
GTCCAAGGGCATATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA3710.12593560651063324No Hit
GTGTGTAGTTTCTCTCTTATATAAAATGCCCGCCTTCCACAGAGTATGTA3710.12593560651063324No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT3710.12593560651063324No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3700.1255961574364806No Hit
CTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACT3660.12423836113987No Hit
CCTCCTCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3660.12423836113987No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3650.12389891206571735No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3640.1235594629915647No Hit
CAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATG3640.1235594629915647No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3630.12322001391741205No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3620.1228805648432594No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTC3610.12254111576910674No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3590.12186221762080145No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTAAGTACAAT3490.11846772687927494No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG3450.11710993058266433No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3440.11677048150851169No Hit
GGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC3420.11609158336020639No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3400.1154126852119011No Hit
TCACCATACAGCACTCAGGCAAGCTATTGTCTGTTGGGGTGAGTTGATGA3350.11371543984113784No Hit
TCATTAACCTGGTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG3350.11371543984113784No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTGGTCTCCTCC3280.11133929632206928No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3270.11099984724791663No Hit
CCCTTGGACAAAGGCATTAAACCATATTATCCTGAACATGCAGTTAATCA3270.11099984724791663No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3260.11066039817376398No Hit
GGGCACATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATC3260.11066039817376398No Hit
GCGTGAAGTTTCTCTCTTATATAAAATGCCCGCCTTCCACAGAGTATGTA3260.11066039817376398No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC3190.10828425465469543No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT3190.10828425465469543No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCA3170.10760535650639012No Hit
CCTCACCATACAGCACTCAGGCAAGCTATTGTCTGTTGGGGTGAGTTGAT3160.10726590743223748No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT3100.10522921298732157No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3070.10421086576486362No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3070.10421086576486362No Hit
ATACAGCACTCAGGCAAGCTATTGTCTGTTGGGGTGAGTTGATGAATCTG3050.10353196761655832No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC3030.10285306946825302No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTAAGT3020.10251362039410036No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC3010.10217417131994772No Hit
ACTTCACGCAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA3010.10217417131994772No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCATATATTTGGTA2990.10149527317164242No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA2990.10149527317164242No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2980.10115582409748977No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2970.10081637502333711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)