FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349909
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13600.388672483417117No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCTGAGAGTCCAA8700.24863607395065576No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8620.2463497652246727No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7950.22720192964456473No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6820.19490781889005426No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6590.18833468130285302No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT5900.1686152685412493No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5710.16318528531703957No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG5300.15146795309637648No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5130.14660954705366253No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5110.14603796987216677No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5010.14318008396468795No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC4900.1400364094664613No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTGTTCG4650.13289169469776427No Hit
ATATTATCATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC4390.12546119133831937No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA4250.12146015106784906No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4240.1211743624771012No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4220.12060278529560542No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4060.11603016784363936No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA4010.11460122488989993No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3900.11145755039167327No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3860.11031439602868175No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3800.10859966448419447No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA3770.10774229871195083No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3730.1065991443489593No Hit
ATTGACAACAGTGCCAGCAGCACCACCTCCTGCCTCCACCAATCGGCAGT3600.10288389266923686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)