FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349909
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13550.3872435404633776No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9170.2620681377158061No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCTGAGAGTCCAA8580.2452066108616812No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8180.233775067231766No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6850.19576518466229792No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6410.1831904866693912No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5890.1683294799505014No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT5870.16775790276900565No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5800.1657573826337705No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG5040.1440374497369316No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5010.14318008396468795No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC4920.14060798664795704No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4700.13432063765150368No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA4330.1237464597938321No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4330.1237464597938321No Hit
ATATTATCATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC4290.1226033054308406No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4270.12203172824934484No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4270.12203172824934484No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTGTTCG4240.1211743624771012No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA4180.11945963093261391No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA4070.11631595643438722No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4030.11517280207139571No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3780.1080280873026987No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC3640.10402704703222837No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3640.10402704703222837No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3610.10316968125998474No Hit
ATTGACAACAGTGCCAGCAGCACCACCTCCTGCCTCCACCAATCGGCAGT3540.1011691611247496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)