FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328660
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33221.0107710095539462No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG10270.31248098338708696No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9270.2820544027262216No Hit
ATCCTACCCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT8070.24554250593318322No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6860.20872634333353618No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5780.1758656362198016No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA5400.16430353556867278No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTTAGTACAAT5130.15608835879023916No Hit
GGGTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4830.14696038459197955No Hit
TCCTCACATTCATTTACAGGAGGACATTATTGATAGATGTCAACAATATG4770.14513478975232763No Hit
GTTCTATCCTACCCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4490.13661534716728535No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT4260.12961723361528632No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA4040.12292338586989593No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3990.12140205683685268No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC3850.11714233554433152No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3760.11440394328485365No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3620.1101442219923325No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGTTTGATTTTTAGTACAATA3580.1089271587658979No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCA3520.10710156392624597No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA3510.10679729811963733No Hit
ATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGT3400.10345037424694212No Hit
ACACCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGG3370.10253757682711617No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3340.10162477940729021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)