FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527891
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA49220.9323894516102755No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT20850.3949679005703829No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT20410.38663284655354985No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT16950.3210890126939084No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC15510.293810654093364No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15130.28661219835155366No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14720.27884544347223195No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14320.2712681216387474No Hit
CACTAATATGGGCCTAAAGTTTCGGCAACTATTGTGGTTTCACATTTCTT13800.26141760325521746No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC13770.2608493041177061No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAATCT13710.25971270584268347No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13680.2591444067051721No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT13620.2580078084301494No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC13070.24758899090910813No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAA13050.2472101248174339No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12860.24361089694652874No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT12600.23868563775476379No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT12060.22845625327955962No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG11670.22106836449191217No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG11650.2206894984002379No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC11620.22012119926272658No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10990.2081869173749884No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10870.20591372082494303No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10700.2026933590457121No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT10590.20060959554150382No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC10310.19530547025806463No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT10250.19416887198304195No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA10230.19379000589136774No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT9570.1812874248661182No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9490.1797719604994213No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9470.17939309440774706No Hit
CTCATAAGGTGGGCAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC9420.17844592917856147No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC9280.1757938665368419No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9210.17446783521598208No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC9110.17257350475761093No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9060.17162633952842538No Hit
GGACTATCCCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCG8750.16575391510747484No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8610.16310185246575523No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8540.16177582114489544No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT8460.16026035677819853No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGCAACTTTACGGGG8330.15779772718231605No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA8320.15760829413647895No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT8290.1570399949989676No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA8280.15685056195313046No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG8270.15666112890729336No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA8180.15495623149475934No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC8090.1532513340822253No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7970.15097813753217995No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC7970.15097813753217995No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7910.14984153925715726No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC7880.1492732401196459No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTTCGGCAACTATTGTGGTTT7870.14908380707380878No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA7870.14908380707380878No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7840.14851550793629745No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA7810.14794720879878612No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7810.14794720879878612No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG7750.14681061052376343No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT7740.1466211774779263No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7520.1424536504695098No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT7440.1409381861028129No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7310.13847555650693041No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC7270.13771782432358196No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA7220.1367706590943964No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT7210.13658122604855927No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7150.13544462777353658No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7090.1343080294985139No Hit
CCCAACAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTT7050.13355029731516543No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT7030.1331714312234912No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6990.13241369904014277No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6980.13222426599430565No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG6940.1314665338109572No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCCATGTCAACGACCGACCT6890.1305193685817716No Hit
GAAACGATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGA6890.1305193685817716No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC6880.1303299355359345No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6790.12862503812340048No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6780.12843560507756335No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6780.12843560507756335No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6710.12710957375670356No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA6660.126162408527518No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG6610.12521524329833242No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA6580.12464694416082107No Hit
ATACTGCACTCAGGCAAGCCATTCTTTGCTGGGGGGAACTAATGACTCTA6560.12426807806914685No Hit
CCTCAAGGTCGGTCGTTGACATGGCAGAGAGTCCAAGAGTCCTCTTATGT6560.12426807806914685No Hit
GTCGTTGACATGGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG6460.12237374761077571No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6450.1221843145649386No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA6380.1208582832440788No Hit
ACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTTCGGCAA6370.12066885019824168No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6290.11915338583154476No Hit
CTCTTGGACTCTCTGCCATGTCAACGACCGACCTTGAGGCATACTTCAAA6290.11915338583154476No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6240.1182062206023592No Hit
GGATAGTCCCAAGCGGCCCCGAGAAGGGTCGTCCGCGGGATTCAGCGCCG6200.11744848841901073No Hit
CTCCGAAAGACACCAAATACTCTATGACTGTTTCTCTTCCAAAAGTGAGA6180.11706962232733652No Hit
ATGTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA6130.11612245709815094No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT6130.11612245709815094No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT6120.11593302405231384No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC6050.11460699273145403No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC6020.11403869359394268No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6010.11384926054810558No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6010.11384926054810558No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC5980.11328096141059424No Hit
CCTAATAATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACA5920.11214436313557155No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTTTGCTGGGGGGAACTAATGA5760.10911343440217773No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5760.10911343440217773No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC5750.1089240013563406No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5720.10835570221882926No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5710.10816626917299216No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA5700.10797683612715504No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5690.10778740308131791No Hit
GCTTGGGACTATCCCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG5640.10684023785213234No Hit
TAATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG5580.10570363957710965No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5580.10570363957710965No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5560.10532477348543544No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5530.10475647434792411No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG5530.10475647434792411No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA5530.10475647434792411No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAGAGA5510.10437760825624987No Hit
CCTAATACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC5510.10437760825624987No Hit
GTCCCAAGCGGCCCCGAGAAGGGTCGTCCGCGGGATTCAGCGCCGACGGG5470.10361987607290142No Hit
CCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTT5370.10172554561453026No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGATCCTTAT5340.10115724647701892No Hit
TTGTAGGCCCACTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATG5330.10096781343118182No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT5320.1007783803853447No Hit
CCAAATACTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA5280.10002064820199624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)