FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601339
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24510.40759039410382497No Hit
CTTAAAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTGGT17050.28353391348307694No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15530.2582569898177235No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG14820.2464500057371965No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG14790.2459511190859066No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA14550.24196002587558765No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT14010.23298006615236994No Hit
TTCCCCCACTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC13700.2278249040890413No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC13660.22715972188732145No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA13520.22483158418130206No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG12500.20786943803744645No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT12030.20005354716723847No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA11950.1987231827637988No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11620.1932354295996102No Hit
ATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA11240.18691619868327183No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11170.18575212983026212No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC10930.18176103661994317No Hit
CTTATATAGAATGCCAGACTTCCACAGAGTATGTAAATAATGCCTAGTTT10740.17860142116177397No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10670.17743735230876428No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10520.1749429190523149No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10480.17427773685059508No Hit
GTGGAATTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGAGA10320.17161700804371577No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9850.1638011171735078No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9770.16247075277006812No Hit
GATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA9650.16047520616490865No Hit
ATTTACATACTCTGTGGAAGTCTGGCATTCTATATAAGAGAGAAACTACA9630.16014261506404873No Hit
GAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGTGGAGGGGTGAACCCT9520.1583133640093192No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGGAGCACCCACG9360.15565263520243988No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9080.15099635979040107No Hit
CCTTTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACATT9060.15066376868954118No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC8970.14916710873567157No Hit
ATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATTTACAT8860.14733785768094204No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC8810.14650637992879226No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA8660.14401194667234288No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8540.14201640006718338No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8360.13902308015944417No Hit
TTGTTCACATCTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGG8200.13636235135256486No Hit
GTTCAGGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA8080.13436680474740537No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA8070.1342005091969754No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC8070.1342005091969754No Hit
TCCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8040.13370162254568554No Hit
GTTCACATCTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAG7470.12422277617117798No Hit
ACCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACAT7470.12422277617117798No Hit
ATACATAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT7440.12372388951988812No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA7330.12189463846515858No Hit
CTCTTATATAGAATGCCAGACTTCCACAGAGTATGTAAATAATGCCTAGT7330.12189463846515858No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG7270.12089686516257885No Hit
CTGTAAGAGGGCCCACATATTGTTCACATCTATTAATAATGTCCTCCTGT7240.12039797851128899No Hit
ATATAGGCCTCTCACCCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG6940.11540911199839027No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6900.11474392979667043No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6900.11474392979667043No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6890.11457763424624048No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACATT6840.1137461564940907No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG6710.11158431433850124No Hit
ATACCACATCATCCATATAACTGAAAGCCACACAGTGGGGGAAAGCCCTA6700.1114180187880713No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6680.11108542768721137No Hit
TTTACATACTCTGTGGAAGTCTGGCATTCTATATAAGAGAGAAACTACAC6610.10992135883420168No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG6600.10975506328377171No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCTGGTC6600.10975506328377171No Hit
CCTACCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6500.10809210777947215No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6480.10775951667861222No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA6320.10509878787173291No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6320.10509878787173291No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6240.10376842346829326No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6230.10360212791786329No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT6200.10310324126657344No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA6170.10260435461528356No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6130.10193917241356372No Hit
GTTGACAACAGTGCCAGCTGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6130.10193917241356372No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6120.10177287686313377No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTTCTG6080.10110769466141394No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6070.10094139911098399No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA6060.10077510356055402No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA6040.10044251245969413No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6030.10027621690926415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)