FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728301
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA47240.6486329141385224No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27810.38184761520305477No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA25680.3526014656028208No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTGTTCG19440.26692260480213537No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC19110.26239151120209914No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT17920.2460521130686351No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC17900.24577750133529955No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA17620.24193293706860214No Hit
CATGTATACAATCTAGGCAGGCTTTCACCTTCTCGCCAACTTACAAGGCC17130.23520494960188165No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCGAAAGATA17010.23355727920186847No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG16840.23122307946851647No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT16290.22367125680178937No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15200.208704917335003No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG14360.1971712245349107No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC13890.19071784880152573No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA13510.18550022586815068No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA13430.18440177893480855No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12930.1775364856014203No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCTTGAGAGGACAACAGAGT12860.17657534453474594No Hit
GTGTACTTCCTTGAGAGGACAACAGAGTTGTCGGTTCCGATCAGTTTCGC12670.1739665330680584No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT12570.17259347440138076No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC12440.17080849813469978No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG12280.16861160426801555No Hit
GGGTTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAT12130.16655201626799906No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT12110.16627740453466355No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11970.16435512240131483No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11730.16105978160128848No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG11250.15446910000123576No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT11190.15364526480122917No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10850.14897686533452514No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10660.1463680538678376No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG10550.1448576893344922No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT10410.14293540720114348No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT10330.14183696026780138No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG10190.13991467813445266No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA10180.1397773722677849No Hit
GAAATACACCTCCTTTCCATGGCTGCTAGGGTGTGCTGCCAACTGGATCC10090.138541619467775No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10080.13840431360110725No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10040.13785509013443617No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC9870.13552089040108417No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC9860.1353835845344164No Hit
ATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCGAAAGATA9770.13414783173440653No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT9710.13332399653439994No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG9480.13016596160104132No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9440.12961673813437027No Hit
GTGTATTTCCTTGAGAGGACAACAGAGTTGTCGGTTCCGATCAGTTTCGC9350.12838098533436038No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTCTGCCAAGTGTTTGCTG9230.1267333149343472No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9200.12632139733434392No Hit
GCTAAGAGTTCCGCCGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG9180.12604678560100838No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC9100.12494833866766625No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGAAGCTGGATCTTCC9040.12412450346765966No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA9000.1235752800009886No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8940.122751444800982No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAGGCAGGCTTTCACCTT8870.12179030373430766No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8850.12151569200097212No Hit
TGGAAACCACCGTGAACGCCCACCAGGTCTTGCCCAAGGTCTTACATAAG8830.12124108026763661No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8820.12110377440096884No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA8760.12027993920096224No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG8720.11973071573429118No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTCTGCCAAGTG8650.11876957466761683No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA8630.11849496293428129No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA8450.11602345733426152No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA8420.11561153973425824No Hit
GCCTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT8310.11410117520091281No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8280.1136892576009095No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA8160.11204158720089634No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8100.11121775200088975No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA8100.11121775200088975No Hit
GGCTTTCACCTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC8040.11039391680088315No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7970.10943277573420879No Hit
CTATTGTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT7910.1086089405342022No Hit
GGATGGGGCTTGGCCATAGGCCACCAGCGCATGCGTGGAACCTTTGTGGC7850.10778510533419562No Hit
CTCCGAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA7830.10751049360086008No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG7820.10737318773419231No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7800.10709857600085677No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA7790.10696127013418903No Hit
ACTCACCCCAACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAA7720.10600012906751466No Hit
GGTTATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA7710.10586282320084692No Hit
GTTAACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT7630.10476437626750479No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT7580.10407784693416595No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG7530.10339131760082713No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7500.10297940000082383No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7420.10188095306748171No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7350.10091981200080735No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGG7340.10078250613413958No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA7330.10064520026747183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)