FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728301
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA39030.5359047976042872No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27760.3811610858697159No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA24190.3321428914693238No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTGTTCG18510.25415315920203324No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG17700.24303138400194424No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA17630.2420702429352699No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT17330.23795106693523696No Hit
CATGTATACAATCTAGGCAGGCTTTCACCTTCTCGCCAACTTACAAGGCC16790.23053655013517763No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCGAAAGATA16420.2254562330684703No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT14890.20444843546830227No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG14760.20266345920162132No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13340.18316602613479865No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13310.18275410853479537No Hit
GAAATACACCTCCTTTCCATGGCTGCTAGGGTGTGCTGCCAACTGGATCC13260.18206757920145653No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT12990.17836032080142689No Hit
CTTCTGCTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTTTACGCGGT12990.17836032080142689No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC12860.17657534453474594No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA12580.17273078026804853No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCTTGAGAGGACAACAGAGT12330.1692981336013544No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC12200.16751315733467342No Hit
GTGTACTTCCTTGAGAGGACAACAGAGTTGTCGGTTCCGATCAGTTTCGC12200.16751315733467342No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC11910.16353128720130825No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG11790.16188361680129507No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT11740.16119708746795625No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11660.16009864053461412No Hit
GGGTTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAT11650.15996133466794635No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG11580.159000193601272No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11470.1574898290679266No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT11030.1514483709345449No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG11010.15117375920120937No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG10850.14897686533452514No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG10750.1476038066678475No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA10270.1410131250677948No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA10150.13936545466778158No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9950.1366193373344263No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA9890.13579550213441968No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT9700.13318669066773217No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9670.13277477306772886No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9660.1326374672010611No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9630.1322255496010578No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9620.13208824373439004No Hit
GCCTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT9560.13126440853438343No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA9500.13044057333437686No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA9500.13044057333437686No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9480.13016596160104132No Hit
TGGAAACCACCGTGAACGCCCACCAGGTCTTGCCCAAGGTCTTACATAAG9430.1294794322677025No Hit
GTGTATTTCCTTGAGAGGACAACAGAGTTGTCGGTTCCGATCAGTTTCGC9350.12838098533436038No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9320.12796906773435707No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTCTGCCAAGTGTTTGCTG9320.12796906773435707No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT9320.12796906773435707No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9260.1271452325343505No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAGGCAGGCTTTCACCTT9220.12659600906767945No Hit
ATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCGAAAGATA9090.12481103280099848No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA9020.12384989173432413No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG8990.12343797413432085No Hit
CTCCGAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA8950.12288875066764977No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTCTGCCAAGTG8940.122751444800982No Hit
GGATGGGGCTTGGCCATAGGCCACCAGCGCATGCGTGGAACCTTTGTGGC8860.12165299786763989No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA8720.11973071573429118No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG8570.11767112773427471No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGAAGCTGGATCTTCC8540.1172592101342714No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8490.11657268080093258No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG8490.11657268080093258No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC8460.11616076320092929No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8390.11519962213425493No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA8300.11396386933424504No Hit
GCTAAGAGTTCCGCCGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG8160.11204158720089634No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8150.11190428133422857No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA8090.11108044613422198No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7960.10929546986754102No Hit
GTGTTGGGTTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG7920.10874624640086997No Hit
CTATTGTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT7890.10833432880086667No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7870.10805971706753115No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG7840.10764779946752785No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGGCTTTTGG7690.10558821146751138No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7660.10517629386750807No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG7620.10462707040083702No Hit
GGCTTTCACCTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC7580.10407784693416595No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA7550.10366592933416267No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7490.10284209413415607No Hit
ACTCACCCCAACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAA7410.10174364720081394No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA7410.10174364720081394No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7400.10160634133414617No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACA7390.10146903546747843No Hit
GTAGAGTCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG7390.10146903546747843No Hit
GTTGTTAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7380.10133172960081066No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7350.10091981200080735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)